||Recombinant human MAP1LC3B2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques(1-120aa).
||Microtubule-associated proteins 1A/1B light chain 3 beta 2, also as known as MAP1LC3B2, belongs to the MAP1LC3 family. MAP1LC3B2 is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. This protein probably involved in formation of autophagosomal vacuoles (autophagosomes). It is expressed primarily in heart, testis, brain and skeletal muscle and testis.
||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT,10% glycerol, 0.1M NaCl
||16.2kDa (140aa) confirmed by MALDI-TOF
||> 95% by SDS - PAGE
||1mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MPSEKTFKQR RTFEQRVEDV RLIREQHPTK IPVIIERYKG EKQLPVLDKT KFLVPDHVNM SELIKIIRRR LQLNANQAFF LLVNGHSMVS VSTPISEVYE SEKDEDGFLY MVCASQETFG
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.