Recombinant E.coli Nth, His-tagged
Cat.No. : | nth-989E |
Product Overview : | Recombinant E.coli nth protein, fused to His-tag at N-terminus, was expressed in E.coliand purified by using conventional chromatography (1-211aa). |
- Specification
- Gene Information
- Related Products
Cat. No. : | nth-989E |
Description : | Nth, also known as Endonuclease III, has an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. This enzyme incises damaged DNA at cytosines, thymines and guanines. Nth acts on a damaged strand, 5" from the damaged site. This enzyme is required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions. |
Form : | Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.1M NaCl, 1mM DTT, 0.1mM PMSF |
Molecular Weight : | 25.7 kDa (231aa) confirmed by MALDI-TOF |
Purity : | > 85% by SDS - PAGE |
Concentration : | 0.5 mg/ml (determined by Bradford assay) |
Sequences of amino acids : | MGSSHHHHHH SSGLVPRGSH MNKAKRLEIL TRLRENNPHP TTELNFSSPF ELLIAVLLSA QATDVSVNKA TAKLYPVANT PAAMLELGVE GVKTYIKTIG LYNSKAENII KTCRILLEQH NGEVPEDRAA LEALPGVGRK TANVVLNTAF GWPTIAVDTH IFRVCNRTQF APGKNVEQVE EKLLKVVPAE FKVDCHHWLI LHGRYTCIAR KPRCGSCIIE DLCEYKEKVD I |
Storage : | Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles. |
Gene Name : | nth DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III) [ Escherichia coli str. K-12 substr. MG1655 ] |
Synonyms : | nth; ECK1629; JW1625; DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III); NP_416150.1; EC 4.2.99.18;endonuclease III; specific for apurinic and/or apyrimidinic sites |
Gene ID : | 947122 |
Protein Refseq : | NP_416150 |
Products Types
◆ Recombinant Protein | ||
NTH-0564B | Recombinant Bacillus subtilis NTH protein, His-tagged | +Inquiry |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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