Recombinant E.coli Nth, His-tagged

• Cat.No.: nth-989E
• Product Overview: Recombinant E.coli nth protein, fused to His-tag at N-terminus, was expressed in E.coliand purified by using conventional chromatography (1-211aa).

Specification

• Species: E.coli
• Source: E.coli
• Tag: His
• Description: Nth, also known as Endonuclease III, has an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. This enzyme incises damaged DNA at cytosines, thymines and guanines. Nth acts on a damaged strand, 5" from the damaged site. This enzyme is required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.1M NaCl, 1mM DTT, 0.1mM PMSF
• Molecular Weight: 25.7 kDa (231aa) confirmed by MALDI-TOF
• Purity: > 85% by SDS - PAGE
• Concentration: 0.5 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MNKAKRLEIL TRLRENNPHP TTELNFSSPF ELLIAVLLSA QATDVSVNKA TAKLYPVANT PAAMLELGVE GVKTYIKTIG LYNSKAENII KTCRILLEQH NGEVPEDRAA LEALPGVGRK TANVVLNTAF GWPTIAVDTH IFRVCNRTQF APGKNVEQVE EKLLKVVPAE FKVDCHHWLI LHGRYTCIAR KPRCGSCIIE DLCEYKEKVD I
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: nth DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III) [ Escherichia coli str. K-12 substr. MG1655 ]
• Synonyms: nth; ECK1629; JW1625; DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III); NP_416150.1; EC 4.2.99.18;endonuclease III; specific for apurinic and/or apyrimidinic sites
• Gene ID: 947122
• Protein Refseq: NP_416150