Active Recombinant Human CD19 protein, His-tagged

• Cat.No.: CD19-3309H
• Product Overview: Recombinant Human CD19 protein( Pro 20 - Lys 291), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Monoclonal Anti-Human CD19 Antibody, Mouse IgG2a at 2 μg/mL (100 μL/well) can bind Human CD19 (20-291), His Tag with a linear range of 10-78 ng/mL.Immobilized Human CD19 (20-291), His Tag at 2 μg/mL (100 μL/well) can bind different Anti-CD19 antibodies with high affinity.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 32.0 kDa. The protein migrates as 47-58 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein Length: Pro 20 - Lys 291
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publication:
  Sensitive and adaptable pharmacological control of CAR T cells through extracellular receptor dimerization (2019)

Gene Information

• Gene Name: CD19 CD19 molecule [ Homo sapiens ]
• Official Symbol: CD19
• Synonyms: CD19; CD19 molecule; CD19 antigen; B-lymphocyte antigen CD19; differentiation antigen CD19; T-cell surface antigen Leu-12; B-lymphocyte surface antigen B4; B4; CVID3; MGC12802
• Gene ID: 930
• mRNA Refseq: NM_001178098
• Protein Refseq: NP_001171569
• MIM: 107265
• UniProt ID: P15391