Mouse Anti-Human Cyclin A1 Monoclonal Antibody


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Mouse Anti-Human Cyclin A1 Monoclonal Antibody

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Cat.No. : CAB11491MH
Product Overview : Mouse monoclonal antibody to human cyclin A1.
Antigen Description : Cyclin-A1, also known as CCNA1, is a nucleus protein which belongs to the cyclin family and Cyclin AB subfamily. The expression of Cyclin-A1 (CCNA1) is increased in early G1 phase and reaches highest levels during the S and G2/M phases. The mammalian A-type cyclin family consists of two members, Cyclin-A1 (CCNA1) and Cyclin-A2 (CCNA2). CCNA2 promotes both G1/S and G2/M transitions, and targeted deletion of Ccna2 in mouse is embryonic lethal Cyclin-A1 (CCNA1) is expressed in mice exclusively in the germ cell lineage and is expressed in humans at highest levels in the testis and certain myeloid leukaemia cells. Cyclin-A1 (CCNA1) is essential for spermatocyte passage into the first meiotic division in male mice, a function that cannot be complemented by the concurrently expressed B-type cyclins. Cyclin-A1 (CCNA1) may be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. Cyclin-A1 (CCNA1) may function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells. Cyclin-A1 (CCNA1) interacts with the CDK2 and the CDC2 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex.
Specificity : Human CCNA1 / Cyclin-A1. No cross-reactivity with Baculovirus lysate in ELISA.
Immunogen : Recombinant human CCNA1 protein
Host : Mouse
Isotype : Mouse IgG2a
Species : Human
Clone : 4G7C3D3
Purification : Protein A affinity chromatography
Applications : Western Blot; ELISA
Dilution : Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human CCNA1 in WB. Using a DAB detection system, the detection limit for human CCNA1 is approximately 8 ng/lane under non-reducing conditions and 4 ng/lane under reducing conditions.Direct ELISA: This antibody can be used at 0.5 - 1 μg/mL with the appropriate secondary reagents to detect human CCNA1. The detection limit for human CCNA1 is 0.0195 ng/well.
Preparation : This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, insect cell-derived, recombinant human CCNA1. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Format : 0.2 μm filtered solution in PBS with 5% trehalose
Storage : This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 °C to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Gene Name : CCNA1 cyclin A1 [ Homo sapiens ]
Official Symbol : CCNA1
Synonyms : CCNA1; cyclin A1; cyclin-A1;
Gene ID : 8900
mRNA Refseq : NM_001111045
Protein Refseq : NP_001104515
MIM : 604036
UniProt ID : P78396
Chromosome Location : 13q12.3-q13
Pathway : APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; Acute myeloid leukemia, organism-specific biosystem; Acute myeloid leukemia, conserved biosystem; C-MYB transcription factor network, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Cell cycle, organism-specific biosystem;
Function : protein binding; protein kinase binding;

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