|Cat. No. :
|Product Overview :
||Recombinant human CDCA8 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (19-280aa).
||CDCA8, also known as Borealin, is a component of the chromosomal passenger complex (CPC). The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly.In the complex, CDCA8 protein may be required to direct the CPC to centromeric DNA. Deletion of CDCA8 delays mitotic progression and causes kinetochore-spindle to mis-attach.
||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT,50% glycerol, 1mM EDTA, 0.2M NaCl
|Molecular Weight :
||31.6 kDa (283aa)confirmed by MALDI-TOF
||> 90% by SDS - PAGE
||0.25 mg/ml (determined by Bradford assay)
|Sequences of amino acids :
||MGSSHHHHHH SSGLVPRGSH MRKLASFLKD FDREVEIRIK QIESDRQNLL KEVDNLYNIE ILRLPKALRE MNWLDYFALG GNKQALEEAATADLDITEIN KLTAEAIQTP LKSAKTRKVI QVDEMIVEEE EEEENERKNL QTARVKRCPP SKKRTQSIQG KGKGKRSSRA NTVTPAVGRLEVSMVKPTPG LTPRFDSRVF KTPGLRTPAA GERIYNISGN GSPLADSKEI FLTVPVGGGE SLRLLASDLQ RHSIAQLDPE ALGNIKKLSNRLAQICSSIR THK
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.