||Recombinant human SPSB1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (24-233aa).
||SPRY domain-containing SOCS box protein 1(SPSB1), also known as SSB1, is a member of the SOCS box protein subfamily. This protein contains a central SPRY domain and a C-terminal SOCS box. Although some of the SOCS protein subfamilies function as adaptors for a large family of ubiquitin-protein isopeptide ligases to regulate certain signaling pathways, the function of the SSB subfamily remains to be determined. SPSB1may play an important role in enhancing the HGF-induced Erk-Elk-1-SRE pathway.Over expression of SPSB1 exhibited no effect on the basal level or epidermal growth factor-induced SRE-luciferase activity.
||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT,30% glycerol, 0.1M NaCl
||26.1 kDa (231aa)confirmed by MALDI-TOF
||> 90% by SDS - PAGE
||1 mg/ml (determined by Bradford assay )
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MQELQGLDYC KPTRLDLLLD MPPVSYDVQL LHSWNNNDRS LNVFVKEDDK LIFHRHPVAQ STDAIRGKVGYTRGLHVWQI TWAMRQRGTH AVVGVATADA PLHSVGYTTL VGNNHESWGW DLGRNRLYHD GKNQPSKTYP AFLEPDETFI VPDSFLVALDMDDGTLSFIV DGQYMGVAFR GLKGKKLYPV VSAVWGHCEI RMRYLNGLDP E
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.