Recombinant Human SPSB1, His-tagged

• Cat.No.: SPSB1-3528H
• Product Overview: Recombinant human SPSB1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (24-233aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: SPRY domain-containing SOCS box protein 1(SPSB1), also known as SSB1, is a member of the SOCS box protein subfamily. This protein contains a central SPRY domain and a C-terminal SOCS box. Although some of the SOCS protein subfamilies function as adaptors for a large family of ubiquitin-protein isopeptide ligases to regulate certain signaling pathways, the function of the SSB subfamily remains to be determined. SPSB1may play an important role in enhancing the HGF-induced Erk-Elk-1-SRE pathway.Over expression of SPSB1 exhibited no effect on the basal level or epidermal growth factor-induced SRE-luciferase activity.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT,30% glycerol, 0.1M NaCl
• Molecular Weight: 26.1 kDa (231aa)confirmed by MALDI-TOF
• Purity: > 90% by SDS - PAGE
• Concentration: 1 mg/ml (determined by Bradford assay )
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MQELQGLDYC KPTRLDLLLD MPPVSYDVQL LHSWNNNDRS LNVFVKEDDK LIFHRHPVAQ STDAIRGKVGYTRGLHVWQI TWAMRQRGTH AVVGVATADA PLHSVGYTTL VGNNHESWGW DLGRNRLYHD GKNQPSKTYP AFLEPDETFI VPDSFLVALDMDDGTLSFIV DGQYMGVAFR GLKGKKLYPV VSAVWGHCEI RMRYLNGLDP E
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: SPSB1 splA/ryanodine receptor domain and SOCS box containing 1 [ Homo sapiens ]
• Synonyms: SPSB1; SSB1; SSB-1; SPRY domain-containing SOCS box protein 1; novel SPRY domain containing protein; SPRY domain-containing SOCS box protein SSB-1
• Gene ID: 80176
• mRNA Refseq: NM_025106
• Protein Refseq: NP_079382
• MIM: 611657
• UniProt ID: Q96BD6
• Chromosome Location: 1p36.22