Recombinant Human DIM1 Dimethyladenosine Transferase 1 Homolog (S. cerevisiae), His-tagged

• Cat.No.: DIMT1-2513H
• Product Overview: Recombinant human DIMT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Description: DIMT1 belongs to the methyltransferase superfamily. This enzyme is specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3"-end of 18S rRNA in the 40S particle. It functions to dimethylate adjacent adenosines on the conserved hairpin loop of 18S rRNA in the 40S particle. DIMT1L is localized to the nucleolus.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20 mM Tris-HCl buffer, pH8.0, 30% glycerol, 2mM DTT, 200mM NaCl, 2mM EDTA.
• Molecular Mass: 37.5 kDa (334aa), confirmed by MALDI-TOF
• Protein length: 1-313aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MMPKVKSGAI GRRRGRQEQR RELKSAGGLM FNTGIGQHIL KNPLIINSII DKAALRPTDV VLEVGPGTGN MTVKLLEKAK KVVACELDPR LVAELHKRVQ GTPVASKLQV LVGDVLKTDL PFFDTCVANL PYQISSPFVF KLLLHRPFFR CAILMFQREF ALRLVAKPGD KLYCRLSINT QLLARVDHLM KVGKNNFRPP PKVESSVVRI EPKNPPPPIN FQEWDGLVRI TFVRKNKTLS AAFKSSAVQQ LLEKNYRIHC SVHNIIIPED FSIADKIQQI LTSTGFSDKR ARSMDIDDFI RLLHGFNAEG IHFS
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: DIMT1 DIM1 dimethyladenosine transferase 1 homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: DIMT1
• Synonyms: DIMT1; DIM1 dimethyladenosine transferase 1 homolog (S. cerevisiae); DIM1 dimethyladenosine transferase 1 like (S. cerevisiae) , DIMT1L; probable dimethyladenosine transferase; HSA9761; 18S rRNA dimethylase; dimethyladenosine transferase; probable 18S rRNA dimethylase; S-adenosylmethionine-6-N,N-adenosyl(rRNA) dimethyltransferase; 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase; probable S-adenosylmethionine-6-N,N-adenosyl(rRNA) dimethyltransferase; probable 18S rRNA (adenine(1779)-N(6)/adenine(1780)-N(6))-dimethyltransferase; DIM1; DIMT1L; HUSSY5
• Gene ID: 27292
• mRNA Refseq: NM_014473
• Protein Refseq: NP_055288
• MIM: 612499
• UniProt ID: Q9UNQ2
• Chromosome Location: 5q12.1
• Function: RNA binding; methyltransferase activity; rRNA (adenine-N6,N6-)-dimethyltransferase activity; transferase activity