Recombinant Human Acyl-CoA Dehydrogenase, Short/Branched Chain, His-tagged

• Cat.No.: ACADSB-2516H
• Product Overview: Recombinant human ACADSB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Description: ACADSB is a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. ACADSB catalyzes the degradation of L-isoleucine and has the highest affinity for (s)-2-methylbutyryl-CoA, isobutyryl-CoA and 2-methylhexanoyl-CoA as substrates.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT.
• Molecular Mass: 46.4 kDa (424aa)
• Protein length: 34-432aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMKSSQS EALLNITNNG IHFAPLQTFT DEEMMIKSSV KKFAQEQIAP LVSTMDENSK MEKSVIQGLF QQGLMGIEVD PEYGGTGASF LSTVLVIEEL AKVDASVAVF CEIQNTLINT LIRKHGTEEQ KATYLPQLTT EKVGSFCLSE AGAGSDSFAL KTRADKEGDY YVLNGSKMWI SSAEHAGLFL VMANVDPTIG YKGITSFLVD RDTPGLHIGK PENKLGLRAS STCPLTFENV KVPEANILGQ IGHGYKYAIG SLNEGRIGIA AQMLGLAQGC FDYTIPYIKE RIQFGKRLFD FQGLQHQVAH VATQLEAARL LTYNAARLLE AGKPFIKEAS MAKYYASEIA GQTTSKCIEW MGGVGYTKDY PVEKYFRDAK IGTIYEGASN IQLNTIAKHI DAEY
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: ACADSB acyl-CoA dehydrogenase, short/branched chain [ Homo sapiens ]
• Official Symbol: ACADSB
• Synonyms: ACADSB; acyl-CoA dehydrogenase, short/branched chain; ACAD7; SBCAD; 2-MEBCAD; short/branched chain specific acyl-CoA dehydrogenase, mitochondrial; 2-methylbutyryl-coenzyme A dehydrogenase; 2-methyl branched chain acyl-CoA dehydrogenase; 2 MEBCAD; 2 methyl branched chain acyl CoA dehydrogenase; 2 methylbutyryl CoA dehydrogenase; 2-methylbutyryl-CoA dehydrogenase; ACDSB_HUMAN; acyl CoA dehydrogenase, short/branched chain; OTTHUMP00000020685; OTTHUMP00000046795; Short/branched chain specific acyl-CoA dehydrogenase; acyl-Coenzyme A dehydrogenase, short/branched chain; 2-methylbutyryl-coenzyme A dehydrogenase; short/branched chain specific acyl-CoA dehydrogenase, mitochondrial; EC 1.3.99
• Gene ID: 36
• mRNA Refseq: NM_001609
• Protein Refseq: NP_001600
• MIM: 600301
• UniProt ID: P45954
• Chromosome Location: 10q26.13
• Pathway: Branched-chain amino acid catabolism, organism-specific biosystem; Fatty acid metabolism, organism-specific biosystem; Fatty acid metabolism, conserved biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem; Valine, leucine and isoleucine degradation, organism-specific biosystem; Valine, leucine and isoleucine degradation, conserved biosystem
• Function: acyl-CoA dehydrogenase activity; electron carrier activity; flavin adenine dinucleotide binding; short-branched-chain-acyl-CoA dehydrogenase activity