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Recombinant Human Acyl-CoA Dehydrogenase, Short/Branched Chain, His-tagged

Cat.No.: ACADSB-2516H
Product Overview: Recombinant human ACADSB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Description: ACADSB is a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. ACADSB catalyzes the degradation of L-isoleucine and has the highest affinity for (s)-2-methylbutyryl-CoA, isobutyryl-CoA and 2-methylhexanoyl-CoA as substrates.
Source: E. coli
Species: Human
Tag: His
Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT.
Molecular Mass: 46.4 kDa (424aa)
Protein length: 34-432aa
AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMKSSQS EALLNITNNG IHFAPLQTFT DEEMMIKSSV KKFAQEQIAP LVSTMDENSK MEKSVIQGLF QQGLMGIEVD PEYGGTGASF LSTVLVIEEL AKVDASVAVF CEIQNTLINT LIRKHGTEEQ KATYLPQLTT EKVGSFCLSE AGAGSDSFAL KTRADKEGDY YVLNGSKMWI SSAEHAGLFL VMANVDPTIG YKGITSFLVD RDTPGLHIGK PENKLGLRAS STCPLTFENV KVPEANILGQ IGHGYKYAIG SLNEGRIGIA AQMLGLAQGC FDYTIPYIKE RIQFGKRLFD FQGLQHQVAH VATQLEAARL LTYNAARLLE AGKPFIKEAS MAKYYASEIA GQTTSKCIEW MGGVGYTKDY PVEKYFRDAK IGTIYEGASN IQLNTIAKHI DAEY
Purity: >90% by SDS - PAGE
Applications: SDS-PAGE
Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Concentration: 0.5 mg/ml (determined by Bradford assay)
Gene Name: ACADSB acyl-CoA dehydrogenase, short/branched chain [ Homo sapiens ]
Official Symbol: ACADSB
Synonyms: ACADSB; acyl-CoA dehydrogenase, short/branched chain; ACAD7; SBCAD; 2-MEBCAD; short/branched chain specific acyl-CoA dehydrogenase, mitochondrial; 2-methylbutyryl-coenzyme A dehydrogenase; 2-methyl branched chain acyl-CoA dehydrogenase; 2 MEBCAD; 2 methyl branched chain acyl CoA dehydrogenase; 2 methylbutyryl CoA dehydrogenase; 2-methylbutyryl-CoA dehydrogenase; ACDSB_HUMAN; acyl CoA dehydrogenase, short/branched chain; OTTHUMP00000020685; OTTHUMP00000046795; Short/branched chain specific acyl-CoA dehydrogenase; acyl-Coenzyme A dehydrogenase, short/branched chain; 2-methylbutyryl-coenzyme A dehydrogenase; short/branched chain specific acyl-CoA dehydrogenase, mitochondrial; EC 1.3.99
Gene ID: 36
mRNA Refseq: NM_001609
Protein Refseq: NP_001600
MIM: 600301
UniProt ID: P45954
Chromosome Location: 10q26.13
Pathway: Branched-chain amino acid catabolism, organism-specific biosystem; Fatty acid metabolism, organism-specific biosystem; Fatty acid metabolism, conserved biosystem; Metabolism, organism-specific biosystem; Metabolism of amino acids and derivatives, organism-specific biosystem; Valine, leucine and isoleucine degradation, organism-specific biosystem; Valine, leucine and isoleucine degradation, conserved biosystem;
Function: acyl-CoA dehydrogenase activity; electron carrier activity; flavin adenine dinucleotide binding; short-branched-chain-acyl-CoA dehydrogenase activity

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