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Recombinant Human Carboxymethylenebutenolidase Homolog (Pseudomonas), His-tagged

Cat.No.: CMBL-6960H
Product Overview: Recombinant human CMBL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Description: CMBL is a cysteine hydrolase of the dienelactone hydrolase family that is highly expressed in liver cytosol. CMBL is the human homolog of Pseudomonas dienelactone hydrolase, a protein that participates in the bacterial halocatechol degradation pathway. CMBL is inhibited by p-chloromercuribenzoate (PCMB) and is encoded by a gene that maps to human chromosome 5p15.2.
Source: E. coli
Species: Human
Tag: His
Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM EDTA.
Molecular Mass: 30.6 kDa (269aa) confirmed by MALDI-TOF
Protein length: 1-245aa
AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMANEAY PCPCDIGHRL EYGGLGREVQ VEHIKAYVTK SPVDAGKAVI VIQDIFGWQL PNTRYIADMI SGNGYTTIVP DFFVGQEPWD PSGDWSIFPE WLKTRNAQKI DREISAILKY LKQQCHAQKI GIVGFCWGGT AVHHLMMKYS EFRAGVSVYG IVKDSEDIYN LKNPTLFIFA ENDVVIPLKD VSLLTQKLKE HCKVEYQIKT FSGQTHGFVH RKREDCSPAD KPYIDEARRN LIEWLNKYM
Purity: >95% by SDS - PAGE
Applications: SDS-PAGE
Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Concentration: 0.5 mg/ml (determined by Bradford assay)
Gene Name: CMBL carboxymethylenebutenolidase homolog (Pseudomonas) [ Homo sapiens ]
Official Symbol: CMBL
Synonyms: CMBL; carboxymethylenebutenolidase homolog (Pseudomonas); carboxymethylenebutenolidase like (Pseudomonas); carboxymethylenebutenolidase homolog; FLJ23617; carboxymethylenebutenolidase-like (Pseudomonas); JS-1;
Gene ID: 134147
mRNA Refseq: NM_138809
Protein Refseq: NP_620164
MIM: 613379
UniProt ID: Q96DG6
Chromosome Location: 5p15.2
Pathway: Metabolic pathways, organism-specific biosystem;
Function: hydrolase activity;

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