Recombinant Human Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 1, 50kDa, His-tagged

• Cat.No.: CSTF1-6966H
• Product Overview: Recombinant human CSTF1 protein, fused to His-tag at C-terminus, was expressed in E. coli.

Specification

• Description: CSTF1, also known as cleavage stimulation factor subunit 1, is involved in the polyadenylation and 3"end cleavage of pre-mRNAs. Similar to mammalian G protein beta subunits, this protein contains transducin-like repeats. Several transcript variants with different 5" UTR, but encoding the same protein, have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 0.1M NaCl, 2M urea.
• Molecular Mass: 49.4 kDa (439aa)
• Protein length: 1-431aa
• AA Sequence: MYRTKVGLKD RQQLYKLIIS QLLYDGYISI ANGLINEIKP QSVCAPSEQL LHLIKLGMEN DDTAVQYAIG RSDTVAPGTG IDLEFDADVQ TMSPEASEYE TCYVTSHKGP CRVATYSRDG QLIATGSADA SIKILDTERM LAKSAMPIEV MMNETAQQNM ENHPVIRTLY DHVDEVTCLA FHPTEQILAS GSRDYTLKLF DYSKPSAKRA FKYIQEAEML RSISFHPSGD FILVGTQHPT LRLYDINTFQ CFVSCNPQDQ HTDAICSVNY NSSANMYVTG SKDGCIKLWD GVSNRCITTF EKAHDGAEVC SAIFSKNSKY ILSSGKDSVA KLWEISTGRT LVRYTGAGLS GRQVHRTQAV FNHTEDYVLL PDERTISLCC WDSRTAERRN LLSLGHNNIV RCIVHSPTNP GFMTCSDDFR ARFWYRRSTT DVEHHHHHH
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: CSTF1 cleavage stimulation factor, 3 pre-RNA, subunit 1, 50kDa [ Homo sapiens ]
• Official Symbol: CSTF1
• Synonyms: CSTF1; cleavage stimulation factor, 3 pre-RNA, subunit 1, 50kDa; cleavage stimulation factor, 3 pre RNA, subunit 1, 50kD; cleavage stimulation factor subunit 1; CF-1 50 kDa subunit; CSTF 50 kDa subunit; cleavage stimulation factor 50 kDa subunit; CstF-50; CstFp50
• Gene ID: 1477
• mRNA Refseq: NM_001033521
• Protein Refseq: NP_001028693
• MIM: 600369
• UniProt ID: Q05048
• Chromosome Location: 20q13.31
• Pathway: BARD1 signaling events, organism-specific biosystem; Cleavage of Growing Transcript in the Termination Region, organism-specific biosystem; Gene Expression, organism-specific biosystem; Processing of Capped Intron-Containing Pre-mRNA, organism-specific biosystem; Processing of Capped Intronless Pre-mRNA, organism-specific biosystem; Processing of Intronless Pre-mRNAs, organism-specific biosystem; RNA Polymerase II Transcription, organism-specific biosystem
• Function: RNA binding; protein binding