Recombinant Human PAD1, GST-tagged

• Cat.No.: PSMD14-6978H
• Product Overview: Recombinant full-length human PAD1 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Description: PAD1 is a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. PAD1 is a component of the 26S proteasome, a multiprotein complex that degrades proteins targeted for destruction by the ubiquitin pathway. The overexpression of PAD1 induced a distinctive pattern of multidrug resistance in mammalian cells and moderate resistance to ultraviolet light. PAD1 is also responsible for substrate deubiquitination during proteasomal degradation.
• Source: Sf9 Cells
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~95 kDa
• Protein length: Full length
• Purity: >95%
• Applications: Western Blot
• Storage: Store at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid freeze/thaw cycles.
• Concentration: 0.1 μg/μ
• Publication:
  Blockade of deubiquitylating enzyme Rpn11 triggers apoptosis in multiple myeloma cells and overcomes bortezomib resistance (2017)

Gene Information

• Gene Name: PSMD14 proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 [ Homo sapiens ]
• Official Symbol: PSMD14
• Synonyms: PSMD14; proteasome (prosome, macropain) 26S subunit, non-ATPase, 14; 26S proteasome non-ATPase regulatory subunit 14; pad1; POH1; Rpn11; 26S proteasome regulatory subunit rpn11; 26S proteasome-associated PAD1 homolog 1; PAD1; RPN11
• Gene ID: 10213
• mRNA Refseq: NM_005805
• Protein Refseq: NP_005796
• MIM: 607173
• UniProt ID: O00487
• Chromosome Location: 2q14.3
• Pathway: APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; APC/C:Cdc20 mediated degradation of Securin, organism-specific biosystem; APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem; APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1, organism-specific biosystem; Activation of APC/C and APC/C:Cdc20 mediated degradation of mitotic proteins, organism-specific biosystem; Activation of NF-kappaB in B Cells, organism-specific biosystem; Adaptive Immune System, organism-specific biosystem
• Function: endopeptidase activator activity; metal ion binding; metallopeptidase activity; peptidase activity; proteasome binding; ubiquitin thiolesterase activity