Recombinant Human ARF5, His-tagged

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Recombinant Human ARF5, His-tagged

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Cat. No. : ARF5-3583H
Product Overview : Recombinant human ARF5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (1-180aa).
Description : ADP-ribosylation factor 5, also known as ARF5, is a small guanine nucleotide-binding protein that enhances the enzymatic activities of cholera toxin. ARF-dependent regulatory mechanisms include the coordination of spectrin interactions with golgi membranes and the association of actin to the golgi via rho family-dependent G-protein localization and WASP/Arp2/3 complexes. ARF5 is involved in vesicular transport and functioning via phospholipase D activation.
Form : Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 0.1M NaCl
Molecular Weight : 22.6 kDa (200aa), confirmed by MALDI-TOF
Purity : > 90% by SDS - PAGE
Concentration : 1 mg/ml (determined by Bradford assay)
Sequences of amino acids : MGSSHHHHHH SSGLVPRGSH MGLTVSALFS RIFGKKQMRI LMVGLDAAGK TTILYKLKLG EIVTTIPTIG FNVETVEYKN ICFTVWDVGG QDKIRPLWRH YFQNTQGLIF VVDSNDRERV QESADELQKM LQEDELRDAV LLVFANKQDM PNAMPVSELT DKLGLQHLRS RTWYVQATCA TQGTGLYDGL DWLSHELSKR
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
PDBrendering based on 1z6x. :

Gene Name : ARF5 ADP-ribosylation factor 5 [ Homo sapiens ]
Official Symbol : ARF5
Synonyms : ARF5; ADP-ribosylation factor 5; EC 3.6.5.3; EC 3.6.5.4; OTTHUMP00000024864; OTTHUMP00000196795; EC 3.6.5.1; EC 3.6.5.2
Gene ID : 381
mRNA Refseq : NM_001662
Protein Refseq : NP_001653
MIM : 103188
UniProt ID : P84085
Chromosome Location : 7q31.3
Function : GTP binding; GTPase activity; nucleotide binding; protein binding

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