Recombinant E.coli ppa, His-tagged

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Recombinant E.coli ppa, His-tagged

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Cat. No. : ppa-3594E
Product Overview : Recombinant E.coli ppa protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (1-176aa).
Description : Inorganic pyrophosphatase (ppa) belongs to the Ppase family. This protein is an enzyme that catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions. This is a highly exergonic reaction, and therefore can be coupled to unfavorable biochemical transformations in order to drive these transformations to completion. The functionality of this enzyme plays a critical role in lipid metabolism (including lipid synthesis and degradation), calcium absorption and bone formation, and DNA synthesis, as well as other biochemical transformations.
Form : Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50mM NaCl
Molecular Weight : 21.9kDa (196aa), confirmed by MALDI-TOF
Purity : > 95% by SDS - PAGE
Concentration : 1mg/ml (determined by Bradford assay)
Sequences of amino acids : MGSSHHHHHH SSGLVPRGSH MSLLNVPAGK DLPEDIYVVI EIPANADPIK YEIDKESGAL FVDRFMSTAM FYPCNYGYIN HTLSLDGDPV DVLVPTPYPL QPGSVIRCRP VGVLKMTDEA GEDAKLVAVP HSKLSKEYDH IKDVNDLPEL LKAQIAHFFE HYKDLEKGKW VKVEGWENAE AAKAEIVASF ERAKNK
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Pathways : Oxidative phosphorylation, organism-specific biosystem; Oxidative phosphorylation, conserved biosystem
Gene Name : ppa inorganic pyrophosphatase [ Escherichia coli str. K-12 substr. MG1655 ]
Official Symbol : ppa
Synonyms : ppa; inorganic pyrophosphatase; EC 3.6.1.1
Gene ID : 948748
Protein Refseq : NP_418647

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