Recombinant Human IL17 protein(Ile20-Ala155)

• Cat.No.: IL17A-7335H
• Product Overview: Recombinant Human IL17A (Q16552) (Ile20-Ala155) was expressed in E. coli, with a N-terminal Met.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Protein Length: 20-155 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells in the presense of 20 ng/mL TNFα. The ED50for this effect is 0.5-2.5 ng/mL.
• Molecular Mass: The recombinant human IL17A consists of 137 amino acids and predicts a molecular mass of 15.7 KDa.
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.