||Recombinant Human IGF-II produced in E.coli is a single, non-glycosylated, polypeptide chain containing 67 amino acids and having a molecular mass of 7.505 kDa.
||This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilmstumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5'' region overlaps the INS gene and the 3'' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
||The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Tyr-Arg-Pro-Ser.
||Purity > 97% by (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver-Stained gel.
||rHuIGF-II is fully biologically active when compared to standards.The ED50, calculated by the competitive binding of IGF-II plasma derived to human placental membrane is < 1.0 ng/ml, corresponding to a specific activity of 106 U/mg.
||Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm.2. Analysis by RP-HPLC, using a calibrated solution of IGF-II as a Reference Standard.
||-20oC, avoid repeated freeze and thaw cycles.