Cat.No. : |
BHMT-1232H |
Product Overview : |
Recombinant human BHMT, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
Description : |
This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. |
Source : |
E.coli |
Species : |
Human |
Tag : |
His |
Form : |
Liquid. 20mM Tris pH7.5, 20% glycerol. |
Molecular Mass : |
49.2 kDa (443 aa), confirmed by MALDI-TOF. |
AA Sequence : |
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMPP VGGKKAKKGI LERLNAGEIV IGDGGFVFAL EKRGYVKAGP WTPEAAVEHP EAVRQLHREF LRAGSNVMQT FTFYASEDKL ENRGNYVLEK ISGQEVNEAA CDIARQVADE GDALVAGGVS QTPSYLSCKS ETEVKKVFLQ QLEVFMKKNV DFLIAEYFEH VEEAVWAVET LIASGKPVAA TMCIGPEGDL HGVPPGECAV RLVKAGASII GVNCHFDPTI SLKTVKLMKE GLEAARLKAH LMSQPLAYHT PDCNKQGFID LPEFPFGLEP RVATRWDIQK YAREAYNLGV RYIGGCCGFE PYHIRAIAEE LAPERGFLPP ASEKHGSWGS GLDMHTKPWV RARARKEYWE NLRIASGRPY NPSMSKPDGW GVTKGTAELM QQKEATTEQQ LKELFEKQKF KSQ |
Purity : |
> 95% by SDS - PAGE |
Applications : |
SDS-PAGE |
Storage : |
Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
Concentration : |
1 mg/ml (determined by Bradford assay) |