| Cat. No. : |
CTGF-555H |
| Description : |
CTGF belongs to the CCN family of secreted proteins. CTGF is a member of cysteine rich regulatory proteins playing an important role in mitogenic and chemotaxis. Each protein has an IGF-binding domain, a thrombospoindin type 1 domain and cysteine knot region. |
| Species : |
Human |
| Source : |
E.coli |
| Form : |
Sterile filtered and lyophilized from 1.0 mg/ml 10 mM NaAcetate, pH 6.0. |
| Purity : |
Purity Greater than 98% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE. |
| Bio-activity : |
Human CTGF is fully biologically active when compared to standard. Activity is determined by the dose-dependent stimulation of the proliferation of HUVEC cells. The expected ED50 is 0.08 ?C 1.8 μg/ml. |
| Physical Appearance : |
Sterile Filtered white lyophilized (freeze-dried) powder. |
| Solubility : |
Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product at a concentration of 0.1 mg/ml with 5 mM NaAcetate, pH 6.0. |
| Purity Greater than 98% as determined by : |
Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE. |
| Protein Content determined by : |
UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard. |
| Endotoxin Level : |
Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug. |
| Amino acid sequence : |
MGKKCIRTPK ISKPIKFELS GCTSMKTYRA KFCGVCTDGR CCTPHRTTTL PVEFKCPDGE VMKKNMMFIK TCACHYNCPG DNDIFESLYY RKMYGDMA |
| Storage : |
Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage. |
