Recombinant Human PTN, His-tagtged

• Cat.No.: PTN-3694H
• Product Overview: Total 152 AA. MW: 17.3 kDa. N-Terminal His-tag and thrombin cleavage site 16 AA (highlighted).

Specification

• Cat. No.: PTN-3694H
• Description: PTN (Osteoblast-Specific Factor-1, OSF-1) contains 136 amino acid residues. The sequence is very rich in cationic amino acids (24% of the residues); lysine cluster sequences are found in the N-terminal and C-terminal ends of the structure.
• Source: E. coli
• Species: Human
• Form: Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 0.1 M phosphate buffer, 0.1 M NaCl, PH 7.2.
• Research topic: Bone and cartilage metabolism; Cytokines and chemokines and related molecules
• Purity: >95%
• Reconstitution: Add PBS pH 7.2 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Applications: ELISA; Western blotting
• Quality Control Test: BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein
• Sequences of amino acids: MKHHHHHHHM LVPRGSGKKE KPEKKVKKSD CGEWQWSVCV PTSGDCGLGT REGTRTGAEC KQTMKTQRCK IPCNWKKQFG AECKYQFQAW GECDLNTALK TRTGSLKRAL HNAECQKTVT ISKPCGKLTK PKPQAESKKK KKEGKKQEKM LD
• Storage: Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.

Gene Information

• Gene Name: PTN pleiotrophin [ Homo sapiens ]
• Official Symbol: PTN
• Synonyms: PTN; pleiotrophin; HARP; HBNF; HBGF8; NEGF1; pleiotrophin; HBBM; OSF-1; HB-GAM; HBGF-8; HBNF-1; OTTHUMP00000208521; OTTHUMP00000208522; osteoblast-specific factor 1; heparin-binding brain mitogen; heparin binding growth factor 8; heparin-binding growth factor 8; heparin affin regulatory protein; neurite growth-promoting factor 1; heparin-binding growth-associated molecule; heparin-binding neurite outgrowth-promoting factor 1; pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1)
• Gene ID: 5764
• mRNA Refseq: NM_002825
• Protein Refseq: NP_002816
• MIM: 162095
• UniProt ID: P21246
• Chromosome Location: 7q33
• Pathway: Syndecan-3-mediated signaling events
• Function: growth factor activity; heparin binding; protein phosphatase inhibitor activity