||Recombinant human CUTC protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (1-273aa).
||CUTC is a member of the Cut family and is suggested to be involved in efflux trafficking of cuprous ion. The Cut family was associated with the copper homeostasis and involved in several important metabolisms, such as uptake, storage, delivery, and efflux of copper. Copper is an essential heavy metal trace element that plays important roles in cell physiology.
||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 5mM DTT, 10% glycerol, 200mM NaCl
||31.5kDa (293a), confirmed by MALDI-TOF
||> 95% by SDS - PAGE
||0.5mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MKRQGASSER KRARIPSGKA GAANGFLMEV CVDSVESAVN AERGGADRIE LCSGLSEGGT TPSMGVLQVV KQSVQIPVFV MIRPRGGDFL YSDREIEVMK ADIRLAKLYG ADGLVFGALT EDGHIDKELC MSLMAICRPL PVTFHRAFDM VHDPMAALET LLTLGFERVL TSGCDSSALE GLPLIKRLIE QAKGRIVVMP GGGITDRNLQ RILEGSGATE FHCSARSTRD SGMKFRNSSV AMGASLSCSE YSLKVTDVTK VRTLNAIAKN ILV
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.