| Cat. No. : |
CDK4-1000H |
| Description : |
CDK4 is a member of the cyclin-dependent protein kinases and is involved in the control of cell proliferation during the G1 phase of the cell cycle. CDK4 forms a complex with the D-type cyclins which regulate its activity. CDK4 is inhibited by p16, also known as cyclin-dependent kinase inhibitor-2. CDK4 can mediate phosphorylation of the C-terminal region of RB protein leading to an active transcriptional repression of E2F complex. CDC37 and HSP90 can preferentially associate with the fraction of CDK4 not bound to D-type cyclins. Pharmacologic inactivation of CDC37/HSP90 function leads to reduced stability of CDK4. SMAD3 is a major physiologic substrate of the G1 cyclin-dependent kinases CDK4 and CDK2. |
| Applications : |
Kinase Assay; Western Blot |
| Molecular Weight : |
57 kDa |
| Expression System : |
E. coli |
| Species : |
Human |
| Form : |
Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. |
| Purity : |
> 80 % |
| Concentration : |
0.2 ug/ul |
| Sequences : |
Full-length |
| Storage : |
Store product at -70°C For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |
| Pathways : |
ATF-2 transcription factor network; B Cell Receptor Signaling Pathway; Bladder cancer; Calcineurin-regulated NFAT-dependent transcription in lymphocytes; Cell Cycle; Cell cycle; Chronic myeloid leukemia; Cyclin D associated events in G1; Diabetes pathways; FOXM1 transcription factor network; G1 Phase; G1 to S cell cycle control; Glioma; HTLV-I infection; IL-7 Signaling Pathway |
