Recombinant Human Cofilin 1 (non-muscle), His-tagged

• Cat.No.: CFL1-1580H
• Product Overview: Recombinant full-length human CFL1 was expressed in E. coli cells using an N-terminal His tag.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Cofilin 1 (non-muscle), also known as CFL1, is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus. CFL1 processed proteins are most probably produced by HIV-1 protease digestion.
• Applications: Western Blot
• Molecular Weight: 23 kDa
• Expression System: E. coli
• Form: Recombinant protein stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 2 mM DTT, 25 % glycerol.
• Purity: > 95 %
• Concentration: 0.2 ug/ul
• Sequences: Full-length
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Axon guidance; CDC42 signaling events; CXCR4-mediated signaling events; Fc gamma R-mediated phagocytosis; Formation of Platelet plug; G13 Signaling Pathway; Hemostasis; Pertussis; Platelet Activation; RAC1 signaling pathway; Regulation of Actin Cytoskeleton; RhoA signaling pathway; Sema3A PAK dependent Axon repulsion; Semaphorin interactions

Gene Information

• Gene Name: CFL1 cofilin 1 (non-muscle) [ Homo sapiens ]
• Official Symbol: CFL1
• Synonyms: CFL1; cofilin 1 (non-muscle); CFL; cofilin-1; p18; OTTHUMP00000233679; OTTHUMP00000233680; OTTHUMP00000233681; OTTHUMP00000233682; OTTHUMP00000233740; OTTHUMP00000233741; OTTHUMP00000233742; 18 kDa phosphoprotein; Cofilin-1, non-muscle isoform; Cofilin-1
• Gene ID: 1072
• mRNA Refseq: NM_005507
• Protein Refseq: NP_005498
• MIM: 601442
• UniProt ID: P23528
• Chromosome Location: 11q13
• Function: actin binding; protein binding