| Cat. No. : |
CSNK1E-1007H |
| Description : |
CSNK1E is a member of the CSNK1E family of serine/threonine protein kinases which play an important role in diverse cell processes, including DNA replication and repair. CSNK1E is a regulator of Yes-associated protein (YAP) transcription coactivator which is a key regulator of organ size and a candidate human oncogene. CSNK1E is activated by CCK2R and this then phosphorylates PKD2 at Ser244. Phosphorylation of PKD2 leads to its nuclear accumulation and efficient phosphorylation of nuclear PKD2 substrates in human gastric cancer cells. CSNK1E can phosphorylate topoisomerase (topo) IIalpha at serine-1106 and this regulates the enzyme activity and sensitivity to topo II-targeted drugs. |
| Applications : |
Kinase Assay; Western Blot |
| Molecular Weight : |
72 kDa |
| Expression System : |
Sf9 insect cells using baculovirus |
| Species : |
Human |
| Form : |
Recombinant protein stored in 50 mM Tris-HCl, ph 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. |
| Specific Activity : |
46 nmol/min/mg |
| Purity : |
> 75 % |
| Concentration : |
0.1 ug/ul |
| Sequences : |
Full length |
| Storage : |
Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |
| Pathways : |
Canonical Wnt signaling pathway; Cell Cycle; Centrosome maturation; Circadian Clock; Circadian rhythm – mammal; Circadian rhythm pathway; FoxO family signaling; G2/M Transition; Hedgehog signaling events mediated by Gli proteins; Hedgehog signaling pathway; Loss of Nlp from mitotic centrosomes; Mitotic G2-G2/M phases; Recruitment of mitotic centrosome proteins and complexes; Wnt Signaling Pathway; p53 pathway |
