||Recombinant human CCNG1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
||CCNG1, also known as cyclin-G1, is a member of the cyclin family and contains the cyclin box. It may play a role in growth regulation and be associated with G2/M phase arrest in response to DNA damage. It may be an intermediate by which p53 mediates its role as an inhibitor of cellular proliferation.
||Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 5 mM DTT, 50% glycerol, 0.2 M NaCl.
||36.2 kDa (315 aa) confirmed by MALDI-TOF
||> 85 % by SDS-PAGE
||0.25 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MIEVLTTTDS QKLLHQLNAL LEQESRCQPK VCGLRLIESA HDNGLRMTAR LRDFEVKDLL SLTQFFGFDT ETFSLAVNLL DRFLSKMKVQ PKHLGCVGLS CFYLAVKSIE EERNVPLATD LIRISQYRFT VSDLMRMEKI VLEKVCWKVK ATTAFQFLQL YYSLLQENLP LERRNSINFE RLEAQLKACH CRIIFSKAKP SVLALSIIAL EIQAQKCVEL TEGIECLQKH SKINGRDLTF WQELVSKCLT EYSSNKCSKP NVQKLKWIVS GRTARQLKHS YYRITHLPTI PEMVP
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||Direct p53 effectors; p53 pathway; p53 signaling pathway