Active Recombinant Human EPO protein, Low Endotoxin

• Cat.No.: EPO-01H
• Product Overview: Recombinant Human EPO protein(AAH93628.1)(Ala28-Arg193) was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Protein Length: 28-193 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human EPO, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human EPO R, hFc Tag with the EC50 of 29.7ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 18.39 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.
• Publications:
  • Dual Delivery of BMP2 and VEGF from a modular polycaprolactone scaffold for the treatment of large bone. (2015)
  • Dual Delivery of BMP2 and VEGF from a polycaprolactone/collagen sponge construct to increase bone growth in ectopic sites for flap prefabrication. (2015)
  • Dual Delivery of EPO and BMP2 from a Novel Modular Poly-?-Caprolactone Construct to Increase the Bone Formation in Prefabricated Bone Flaps. (2015)
  • Oxalic Acid Supported Si–18F-Radiofluorination: One-Step Radiosynthesis of N-Succinimidyl 3-(Di-tert-butyl [18F] fluorosilyl) benzoate ([18F] SiFB) for Protein Labeling (2012)

Gene Information

• Gene Name: EPO erythropoietin [ Homo sapiens ]
• Official Symbol: EPO
• Synonyms: EPO; erythropoietin; EP; epoetin; MVCD2; MGC138142
• Gene ID: 2056
• mRNA Refseq: NM_000799
• Protein Refseq: NP_000790
• MIM: 133170
• UniProt ID: P01588