Recombinant Human Erythropoietin EPO-01H

Recombinant Human Erythropoietin


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Recombinant Human Erythropoietin

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Cat. No. : EPO-01H
Product Overview : Recombinant Human EPO is expressed in CHO cells. The mature protein consists of a 165 amino acid polypeptide chain heavily glycosylated at three N-linked and O-linked glycosylation sites yielding a total molecular mass of 35-45kDa. About 40% of the fully glycosylated EPO molecule consists of carbohydrate.
Description : Erythropoietin (EPO) is a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types.
Purity : ≥98% as determined by SDS-PAGE and HPLC analyses
Formulation : Sterile filtered lyophilized powder.
Specific Activity : rHuEPO is fully biologically active when compared to standards. Its specific activity is ≥1.2×105IU/mg.
Endotoxin : Less than 0.1EU/µg determined by LAL test.
Reconstitution : It is recommended to reconstitute the lyophilized rHuEPO in sterile buffer not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storagee : Lyophilized rHuEPO although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Reconstituted rHuEPO aliquots should be stored at –20°C for maximal stability up to three years. Aliquot to avoid repeated freeze-thaw cycles.
Crystal structure of EPO. Available structures:1buy, 1cn4, 1eer :

Publication :
Gene Name : EPO
Synonyms : EP; MGC138142; Epoetin; Erythropoietin Precursor; EPO; erythropoietin
Gene ID : 2056
mRNA Refseq : NM_000799
Protein Refseq : NP_000790
MIM : 133170
UniProt ID : P01588
Chromosome Location : 7q22
Pathway : Cytokine-cytokine receptor interaction; Hematopoietic cell lineage; Jak-STAT signaling pathway
Function : erythropoietin receptor binding IEA ;hormone activity IEA ;protein binding

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