||The active human SUMO-I (the 1-97 animo acid region of the Ubiquitin-like protein SMT3C precursor is also called Ubiquitin-homology domain protein PIC1, Ubiquitin-like protein UBL1, Ubiquitin-related protein SUMO-I, GAP modifying protein-I, GMP-I, and Sentrin-I. The enzyme contains a single polypeptide band of 11 kDa. The predicted molecular weight of hSOMO I is 11 KDa. The The final fraction of enzyme contains single polypeptide band of approximately 20 kDa on SDS PAGE.
||This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene. Alternate transcriptional splice variants encoding different isoforms have been characterized.
||Sterile Filtered clear solution at a concentration of 0.5mg/ml
||Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
||10mM sodium chloride, 100mM imidazole, 0.5mM PMSF, 1mM DTT and 10% glycerol.
||rHuSUMO-I although stable at 14°C for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.