| Description : |
Transcriptional activity is greatly stimulated by promoter-specific activator proteins . These are modular proteins, consisting of a DNA-binding domain and a regulatory (activator) domain . The GAL4 protein of yeast activates the transcription of several genes involved in galactose metabolism. This event requires that GAL4 bind to upstream activation sites with the consensus sequence 5-CGGN5(T/A)N5CCG-3 . A fragment of the GAL4 protein, comprising amino acids 1-147, binds DNA but fails to activate transcription . Linking of an acidic synthetic peptide, forming an ?-helix (AH), to this GAL4 DNA-binding domain, results in a protein with an amphipathic structure . This fusion protein is able to activate transcription of a gene, bearing the GAL4 binding sites in an in vitro transcription system by targeting TFIIB in the pre-initiation complex. |
| Source : |
E. coli |
| Species : |
Saccharomyces cerevisiae |
| Tag : |
His |
| Form : |
20 mM Tris-Cl, pH 7.9, 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA |
| Bio-activity : |
1 unit equals 1 ng of purified protein. 20 units are sufficient for reconstituted transcription assay and 100 units are sufficient for a protein-protein interaction assay. |
| AA Sequence : |
MKLLSSIEQA CDICRLKKLK CSKEKPKCAK CLKNNWECRY SPKTKRSPLT RAHLTEVESR LERLEQLFLL IFPREDLDMI LKMDSLQDIK ALLTGLFVQD NVNKDAVTDR LASVETDMPL TLRQHRISAT SSSEESSNKG QRQLTVSELN ELNELNALLN NN |
| Purity : |
>95% as determined by SDS-PAGE |
| Storage : |
-80 °C |
| Concentration : |
300 μg/ml |
| Shipping : |
Dry Ice |
