Active Recombinant Mouse Wnt5a

• Cat.No.: Wnt5a-2054M
• Product Overview: Recombinant Mouse Wnt5a (Gln38-Lys380) was expressed in CHO Cells.

Specification

• Cat. No.: Wnt5a-2054M
• Description: Protein Wnt-5a is a protein that in humans is encoded by the WNT5A gene. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. The WNT5A is highly expressed in the dermal papilla of depilated skin.It encodes a protein showing 98%, 98%, and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction.
• Source: CHO Cells
• Molecular Mass: 38 kDa
• Predicted N terminal: Gln38
• Formulation: Lyophilized from a 0.2 µm filtered solution in PBS, EDTA and CHAPS.
• Purity: >80%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Activity: Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is typically 0.1 - 0.5 µg/mL, in the presence of 5 ng/mL rmWnt-3a.
• EndotoxinLevel: <1.0 EU per 1 μg of the protein by the LAL method.
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS.
• Storage: 12 months from date of receipt, ­20 to ­70 °C as supplied. Avoid repeated freeze­thaw cycles.
• OfficialSymbol: Wnt5a

Gene Information

• Gene Name: Wnt5a wingless-related MMTV integration site 5A [ Mus musculus ]
• Synonyms: Wnt5a; wingless-related MMTV integration site 5A; Wnt-5a; 8030457G12Rik; protein Wnt-5a
• Gene ID: 22418
• mRNA Refseq: NM_009524
• Protein Refseq: NP_033550
• UniProt ID: P22725
• Chromosome Location: 14 A3; 14 16.8 cM
• Pathway: Basal cell carcinoma; Class B/2 (Secretin family receptors); Melanogenesis; Pathways in cancer; Wnt signaling pathway
• Function: cytokine activity; frizzled binding; frizzled-2 binding; receptor binding

Reviews

11/14/2019

Satisfied with performance, quality.

08/27/2019

Great product, satisfied customer.

02/27/2019

High purity, works well.

Q&As

What role does WNT5A play in cancer biology?

Its role in cancer is complex, acting as a tumor suppressor or promoter depending on context.

How does WNT5A interact with its receptors to initiate signal transduction?

It binds to Frizzled receptors and co-receptors, activating non-canonical Wnt signaling pathways.

In which developmental processes is WNT5A critically involved?

WNT5A is involved in embryonic development, particularly in limb formation and organogenesis.

How is WNT5A implicated in immune system regulation?

WNT5A modulates various immune responses, influencing inflammation and cell migration.

What is the primary role of WNT5A in cellular processes?

WNT5A is crucial in regulating cell proliferation, migration, and differentiation.

What therapeutic strategies are being explored involving WNT5A modulation?

Strategies include developing WNT5A modulators for potential use in cancer therapy and regenerative medicine.

Are there known genetic mutations in WNT5A associated with specific diseases?

Genetic mutations and altered expression of WNT5A are linked to developmental disorders and cancer.