Active Recombinant Mouse Wnt5a

• Cat.No.: Wnt5a-2054M
• Product Overview: Recombinant Mouse Wnt5a (Gln38-Lys380) was expressed in CHO Cells.

Specification

• Species: Mouse
• Source: CHO
• Tag: Non
• Protein Length: 38-380 a.a.
• Description: Protein Wnt-5a is a protein that in humans is encoded by the WNT5A gene. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. The WNT5A is highly expressed in the dermal papilla of depilated skin.It encodes a protein showing 98%, 98%, and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction.
• Molecular Mass: 38 kDa
• Predicted N terminal: Gln38
• Formulation: Lyophilized from a 0.2 µm filtered solution in PBS, EDTA and CHAPS.
• Purity: >80%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Activity: Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is typically 0.1 - 0.5 µg/mL, in the presence of 5 ng/mL rmWnt-3a.
• EndotoxinLevel: <1.0 EU per 1 μg of the protein by the LAL method.
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS.
• Storage: 12 months from date of receipt, ­20 to ­70 °C as supplied. Avoid repeated freeze­thaw cycles.
• OfficialSymbol: Wnt5a

Gene Information

• Gene Name: Wnt5a wingless-related MMTV integration site 5A [ Mus musculus ]
• Synonyms: Wnt5a; wingless-related MMTV integration site 5A; Wnt-5a; 8030457G12Rik; protein Wnt-5a
• Gene ID: 22418
• mRNA Refseq: NM_009524
• Protein Refseq: NP_033550
• UniProt ID: P22725
• Chromosome Location: 14 A3; 14 16.8 cM
• Pathway: Basal cell carcinoma; Class B/2 (Secretin family receptors); Melanogenesis; Pathways in cancer; Wnt signaling pathway
• Function: cytokine activity; frizzled binding; frizzled-2 binding; receptor binding