Recombinant Human CHMP6, His-tagged

• Cat.No.: CHMP6-15851H
• Product Overview: Recombinant human CHMP6 protein, fused to His tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Description: CHMP6, as known as charged multivesicular body protein 6, belongs to the SNF7 family. This Protein is core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 100mM NaCl
• Molecular Mass: 26.1kDa (225aa), confirmed by MALDI-TOF(Molecular size on SDS-PAGE will appear higher)
• Protein length: 1-201aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMGNLFG RKKQSRVTEQ DKAILQLKQQ RDKLRQYQKR IAQQLERERA LARQLLRDGR KERAKLLLKK KRYQEQLLDR TENQISSLEA MVQSIEFTQI EMKVMEGLQF GNECLNKMHQ VMSIEEVERI LDETQEAVEY QRQIDELLAG SFTQEDEDAILEELSAITQE QIELPEVPSE PLPEKIPENV PVKARPRQAE LVAAS
• Purity: >95% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term. For long term storage, aliquot and store at at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: CHMP6 charged multivesicular body protein 6 [ Homo sapiens ]
• Official Symbol: CHMP6
• Synonyms: CHMP6; charged multivesicular body protein 6; chromatin modifying protein 6; FLJ11749; VPS20; hVps20; chromatin-modifying protein 6; vacuolar protein sorting-associated protein 20
• Gene ID: 79643
• mRNA Refseq: NM_024591
• Protein Refseq: NP_078867
• MIM: 610901
• UniProt ID: Q96FZ7
• Chromosome Location: 17q25.3
• Pathway: ESCRT-III complex, organism-specific biosystem; Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem; Endosomal Sorting Complex Required For Transport (ESCRT), organism-specific biosystem; Membrane Trafficking, organism-specific biosystem
• Function: protein N-terminus binding