Recombinant Human CH25H, His-tagged CH25H-11153H

Recombinant Human CH25H, His-tagged


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Recombinant Human CH25H, His-tagged

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Cat.No. : CH25H-11153H
Product Overview : Recombinant Human CH25H protein, fused to His-tag, was expressed in E.coli and purified by Ni-sepharose.
Description : This is an intronless gene that is involved in cholesterol and lipid metabolism. The encoded protein is a membrane protein and contains clusters of histidine residues essential for catalytic activity. Unlike most other sterol hydroxylases, this enzyme is a member of a small family of enzymes that utilize diiron cofactors to catalyze the hydroxylation of hydrophobic substrates.
Source : E.coli
Species : Human
Tag : His
Protein length : 140-272a.a.
Storage : The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
Storage Buffer : 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 300mM Imidazole and 0.7% Sarcosyl, 15%glycerol.
Gene Name : CH25H cholesterol 25-hydroxylase [ Homo sapiens ]
Official Symbol : CH25H
Synonyms : CH25H; cholesterol 25-hydroxylase; h25OH; cholesterol 25-monooxygenase; C25H;
Gene ID : 9023
mRNA Refseq : NM_003956
Protein Refseq : NP_003947
MIM : 604551
UniProt ID : O95992
Chromosome Location : 10q23
Pathway : Bile acid and bile salt metabolism, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; Primary bile acid biosynthesis, organism-specific biosystem; Primary bile acid biosynthesis, conserved biosystem; Synthesis of bile acids and bile salts, organism-specific biosystem;
Function : cholesterol 25-hydroxylase activity; iron ion binding; metal ion binding; steroid hydroxylase activity;

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