Recombinant Human CD58 protein, His-tagged
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|Product Overview :||Recombinant Human CD58 (Phe29-Arg215) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).|
|Description :||CD antigen CD58 is also known as Lymphocyte function-associated antigen 3 (LFA-3). CD58 is a cell adhesion molecule expressed on Antigen Presenting Cells (APC), particularly macrophages. CD58/LFA3 is ligand of the T-lymphocyte CD2 glycoprotein, which binds to CD2 (LFA-2) on T cells and is important in strengthening the adhesion between the T cells and Professional Antigen Presenting Cells. In addition, the LFA-3/CD2 interaction may prime response by both the CD2+ and LFA-3+ cells. Polymorphisms in the CD58 gene are associated with increased risk for multiple sclerosis.|
|Predicted N Terminal :||Phe29|
|Form :||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 22.3 kDa. The protein migrates as 30-45 kDa under reducing (R) condition (SDS-PAGE) due to differtent glycosylation.|
|Protein length :||Phe29-Arg215|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>95% as determined by SDS-PAGE.|
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of CD58-462H :||
Human CD58, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
|Bioactivity-ELISA of CD58-462H :||
Immobilized Human CD2, Fc Tag at 10 μg/mL (100 μL/well) can bind Human CD58, His Tag with a linear range of 0.078-2.5 μg/mL (QC tested).
|Gene Name :||CD58|
|Official Symbol :||CD58|
|Synonyms :||CD58; CD58 molecule; CD58 antigen, (lymphocyte function associated antigen 3), LFA3; lymphocyte function-associated antigen 3; surface glycoprotein LFA-3; CD58 antigen, (lymphocyte function-associated antigen 3); ag3; LFA3; LFA-3; FLJ23181; FLJ43722|
|Gene ID :||965|
|mRNA Refseq :||NM_001144822|
|Protein Refseq :||NP_001138294|
|UniProt ID :||P19256|
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