Recombinant Human CCNO, GST-tagged

• Cat.No.: CCNO-129H
• Product Overview: Recombinant full-length human Cyclin O was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: Cyclin O belongs to the highly conserved cyclin family. These family members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclin O plays an important role in the processes of transcription. Cyclin O also plays an important role in DNA repair and cell cycle progression. Cyclin O localizes exclusively in the nucleus. Cyclin O shows significant uracil-DNA glycosylase activity in which the activity of in vitro translated UDG2 was not as robust as that of the mitochondrial isoform of UNG
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Molecular Mass: ~69 kDa
• Purity: >95% by densitometry
• Applications: Western Blot
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 μg/μl

Gene Information

• Gene Name: CCNO cyclin O [ Homo sapiens ]
• Official Symbol: CCNO
• Synonyms: CCNO; cyclin O; CCNU, cyclin U; cyclin-O; FLJ22422; UDG2; UNG2; cyclin U; cyclin domain containing; cyclin-like uracil-DNA glycosylase; CCNU
• Gene ID: 10309
• mRNA Refseq: NM_021147
• Protein Refseq: NP_066970
• MIM: 607752
• UniProt ID: P22674
• Chromosome Location: 5q11.2
• Pathway: Base Excision Repair, organism-specific biosystem; Base-Excision Repair, AP Site Formation, organism-specific biosystem; Base-free sugar-phosphate removal via the single-nucleotide replacement pathway, organism-specific biosystem; Cleavage of the damaged pyrimidine, organism-specific biosystem; DNA Repair, organism-specific biosystem; Depyrimidination, organism-specific biosystem; Displacement of DNA glycosylase byAPE1, organism-specific biosystem
• Function: protein kinase binding; uracil DNA N-glycosylase activity