Active Recombinant Human CDK18&CCNY, GST-tagged

• Cat.No.: CDK18&CCNY-826H
• Product Overview: Full-length recombinant human PCTK3 and Cyclin Y (2-end) were co-expressed by baculovirus in Sf9 insect cells using N-terminal GST tags.

Specification

• Description: PCTK3 or PCTAIRE protein kinase 3 is a member of the cdc2/cdkx subfamily of the ser/thr family of protein kinases. PCTK3 is thought to play a role in terminally differentiated neurons and it contains an N-terminal extension relative to CDC2. PCTK3 is expressed in a variety of human cell lines and tissues. Elevation in the protein levels of PCTAIRE 3 in the temporal cortex of Alzheimer"s disease relative to control brains, have been observed. It is proposed that PCTAIRE 3 is a paired helical filaments-associated kinase that modulates tau phosphorylation.
• Source: Sf9 insect cells using baculovirus
• Species: Human
• Tag: GST
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 46 nmol/min/mg
• Molecular Mass: PCTK3 ~76 kDa and Cyclin Y ~64 kDa
• Protein length: PCTK3 (Full length) and Cyclin Y (2-end)
• Purity: >70%
• Applications: Kinase Assay, Western Blot
• Stability: 1yr at –70°C from date of shipment
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1ug/ul

Gene Information

• Gene Name: CDK18 cyclin-dependent kinase 18 [ Homo sapiens ]
• Official Symbol: CDK18
• Synonyms: CDK18; cyclin-dependent kinase 18; PCTAIRE protein kinase 3 , PCTK3; PCTAIRE3; PCTAIRE protein kinase 3; PCTAIRE-motif protein kinase 3; cell division protein kinase 18; serine/threonine-protein kinase PCTAIRE-3; PCTK3; PCTAIRE
• Gene ID: 5129
• mRNA Refseq: NM_002596
• Protein Refseq: NP_002587
• MIM: 169190
• UniProt ID: Q07002
• Chromosome Location: 1q31-q32
• Function: ATP binding; cyclin-dependent protein kinase activity; nucleotide binding; protein serine/threonine kinase activity; signal transducer activity