Active Recombinant Human TTBK1, GST-tagged

• Cat.No.: TTBK1-828H
• Product Overview: Recombinant human TTBK1 (1-479) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 1-479
• Description: TTBK1 or tau-tubulin kinase 1 is a recently discovered brain-specific protein kinase involved in tau phosphorylation at Alzheimer’s disease-related sites. Two single nucleotide polymorphisms have been observed in the TTBK1 gene and these may play an important role in the pathogenesis of sporadic late-onset Alzheimer"s disease. TTBK1 levels are upregulated in brains of human Alzheimer" disease (AD) patients compared with age-matched non-AD controls. Overexpression of TTBK1 in transgenic mice leads to increased phosphorylation-related neurofilament aggregation which results in significant age-dependent memory impairment.
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 160 nmol/min/mg
• Molecular Mass: ~96 kDa
• Purity: >90%
• Applications: Kinase Assay, Western Blot
• Stability: 1yr at –70°C from date of shipment
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1ug/ul

Gene Information

• Gene Name: TTBK1 tau tubulin kinase 1 [ Homo sapiens ]
• Official Symbol: TTBK1
• Synonyms: TTBK1; tau tubulin kinase 1; tau-tubulin kinase 1; KIAA1855; brain-derived tau kinase; BDTK; RP3-330M21.4
• Gene ID: 84630
• mRNA Refseq: NM_032538
• Protein Refseq: NP_115927
• UniProt ID: Q5TCY1
• Chromosome Location: 6p21.1
• Function: ATP binding; nucleotide binding; protein serine/threonine kinase activity