Recombinant Human CAPNS1, His-tagged

• Cat.No.: CAPNS1-188H
• Product Overview: CAPNS1, 84-268aa, Human, His tag, E.coli

Specification

• Description: Calpains are a ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. Calpain families have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. Calpain I and II are heterodimeric with distinct large subunits associated with common small subunits, all of which are encoded by different genes. Recombinant human CAPNS1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
• Molecular Mass: 23.8kDa (208aa) confirmed by MALDI-TOF
• Protein length: 84-268aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSRTHYSNI EANESEEVRQ FRRLFAQLAG DDMEVSATEL MNILNKVVTR HPDLKTDGFG IDTCRSMVAV MDSDTTGKLG FEEFKYLWNN IKRWQAIYKQ FDTDRSGTIC SSELPGAFEA AGFHLNEHLY NMIIRRYSDE SGNMDFDNFI SCLVRLDAMF RAFKSLDKDG TGQIQVNIQE WLQLTMYS
• Purity: >95% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: CAPNS1 calpain, small subunit 1 [ Homo sapiens ]
• Official Symbol: CAPNS1
• Synonyms: CAPNS1; calpain, small subunit 1; CAPN4; calpain small subunit 1; 30K; CANP; CANPS; CDPS; CANP small subunit; calpain regulatory subunit; calpain, small polypeptide; calpain 4, small subunit (30K); calcium-dependent protease, small subunit; calcium-dependent protease small subunit 1; calcium-activated neutral proteinase small subunit; CSS1; CALPAIN4
• Gene ID: 826
• mRNA Refseq: NM_001003962
• Protein Refseq: NP_001003962
• MIM: 114170
• UniProt ID: P04632
• Chromosome Location: 19q13.1
• Pathway: Integrin-mediated cell adhesion, organism-specific biosystem
• Function: calcium ion binding; calcium-dependent cysteine-type endopeptidase activity; protein binding; protein heterodimerization activity