Active Recombinant Human KIAA1804, GST-tagged

• Cat.No.: KIAA1804-85H
• Product Overview: Recombinant human MLK4 (1-557) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 1-557 a.a.
• Description: MLK4 or mixed lineage kinase 4 belongs to the superfamily of MAP kinase kinase kinases (MAP3K1) which contain both ser/thr and tyr kinases activity in their catalytic domains. The structure of this kinase family incorporates an N-terminal Src homology (SH3) domain, followed by the kinase domain, a leucine zipper region, and a CDC42 /RAC -interactive binding (CRIB) motif, but divergent C-terminal regions. MLK4 is highly expressed in kidney and pancreas. MLK4 is a negative regulator of TLR4.
• Form: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: The specific activity of MLK4 was determined to be 22 nmol/min/mg as per activity assay protocol.
• Molecular Mass: ?~61 kDa
• AA Sequence: 1-557
• Purity: The purity of MLK4 was determined to be >80% by densitometry, approx.
• Applications: Kinase Assay; Western Blot
• Stability: 1 yr at –70oC from date of shipment.
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 ug/ul

Gene Information

• Gene Name: KIAA1804?mixed lineage kinase 4 [?Homo sapiens?(human) ]
• Official Symbol: KIAA1804
• Synonyms: KIAA1804; MLK4; dJ862P8.3; RP5-862P8.2; mixed lineage kinase 4; mitogen-activated protein kinase kinase kinase MLK4; EC 2.7.11.25
• Gene ID: 84451
• mRNA Refseq: NM_032435
• Protein Refseq: NP_115811
• MIM: 614793
• UniProt ID: Q5TCX8
• Chromosome Location: 1q42
• Function: ATP binding; MAP kinase kinase kinase activity; protein homodimerization activity