"RPA" Related Products

Recombinant Staphylococcus RPA, His-tagged, Biotinylated

Cat.No. : RPA-264S
Product Overview : Biotinylated Protein A (Biotinylated Protein A ) Ala 2 - Cys 293 was labeled with biotin based on Protein A Protein, which was produced in E.coli cells.
Description : Protein A is a 40-60 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus (SPA). SPA binds proteins from many of mammalian species, most notably IgGs, and helps inhibit phagocytic engulfment and acts as an immunological disguise via this type of interaction, thus the bacteria will disrupts opsonization and phagocytosis. It binds with the Fc region of immunoglobulins through interaction with the heavy chain. It couples to a wide variety of reporter molecules including fluorescent dyes, enzyme markers, biotin, colloidal gold and radioactive iodine without affecting the antibody binding site. Recombinant Protein A was developed to increase the specificity of the molecule for IgG and is widely used both in research and bioprocessing.
Source : E.Coli
Species : Staphylococcus
Tag : His
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose are added as protectants before lyophilization.
Molecular Mass : Biotinylated Protein A , fused with the polyhistidine tag at the N-terminus consisting 299 amino acids. DTT-reduced protein migrates as a 33.8 kDa polypeptide .
Endotoxin : Less than 1.0 EU per μg of the Biotinylated Protein A by the LAL method.
Purity : >95% as determined by reduced SDS-PAGE.
Storage : Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

Online Inquiry

Note: There will be extra charge for optional service!

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional requirements on this protein    +Expand

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.