Recombinant Human PPP2CA protein, His-tagged

• Cat.No.: PPP2CA-1913H
• Product Overview: Recombinant Human PPP2CA protein(1-309 aa), fused with His tag, was expressed in E.coli.

Specification

• Source: E.coli
• Species: Human
• Tag: His
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• Protein Length: 1-309 aa
• AA Sequence: MDEKVFTKELDQWIEQLNECKQLSE SQVKSLCEKAKEILTKESNVQEVRC PVTVCGDVHGQFHDLMELFRIGGKS PDTNYLFMGDYVDRGYYSVETVTLL VALKVRYRERITILRGNHESRQITQ VYGFYDECLRKYGNANVWKYFTDLF DYLPLTALVDGQIFCLHGGLSPSID TLDHIRALDRLQEVPHEGPMCDLLW SDPDDRGGWGISPRGAGYTFGQDIS ETFNHANGLTLVSRAHQLVMEGYNW CHDRNVVTIFSAPNYCYRCGNQAAI MELDDTLKYSFLQFDPAPRRGEPHV TRRTPDYFL
• Purity: 90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.
• Publication:
  CDC25B partners with PP2A to induce AMPK activation and tumor suppression in triple negative breast cancer (2020)

Gene Information

• Gene Name: PPP2CA protein phosphatase 2, catalytic subunit, alpha isozyme [ Homo sapiens ]
• Official Symbol: PPP2CA
• Synonyms: PPP2CA; protein phosphatase 2, catalytic subunit, alpha isozyme; protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform; serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; PP2Calpha; protein phosphatase 2A catalytic subunit; alpha isoform; PP2A-alpha; replication protein C; protein phosphatase 2A catalytic subunit, alpha isoform; serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform; RP-C; PP2Ac; PP2CA
• Gene ID: 5515
• mRNA Refseq: NM_002715
• Protein Refseq: NP_002706
• MIM: 176915
• UniProt ID: P67775