Recombinant Human eukaryotic translation initiation factor 3, subunit F, His-tagged EIF3F-274H

Recombinant Human eukaryotic translation initiation factor 3, subunit F, His-tagged

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Recombinant Human eukaryotic translation initiation factor 3, subunit F, His-tagged

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Cat.No. : EIF3F-274H
Product Overview : EIF3F, 1-357 aa, Human, His tag, E.coli
Description : EIF3F is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF 2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Recombinant human EIF3F protein, fused to His-tag at N-terminus, was expressed in E.coli.
Source : E.coli
Species : Human
Tag : His-tag
Form : Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M urea
Molecular Mass : 400kDa (380aa)
AA Sequence : MGSSHHHHHH SSGLVPRGSH MGSMATPAVP VSAPPATPTP VPAAAPASVP APTPAPAAAP VPAAAPASSS DPAAAAAATA APGQTPASAQ APAQTPAPAL PGPALPGPFP GGRVVRLHPV ILASIVDSYE RRNEGAARVI GTLLGTVDKH SVEVTNCFSV PHNESEDEVA VDMEFAKNMY ELHKKVSPNE LILGWYATGH DITEHSVLIH EYYSREAPNP IHLTVDTSLQ NGRMSIKAYV STLMGVPGRT MGVMFTPLTV KYAYYDTERI GVDLIMKTCF SPNRVIGLSS DLQQVGGASA RIQDALSTVL QYAEDVLSGK VSADNTVGRF LMSLVNQVPK IVPDDFETML NSNINDLLMV TYLANLTQSQ IALNEKLVNL
Purity : >90% by SDS - PAGE
Storage : Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Concentration : 1mg/ml (determined by Bradford assay)
Gene Name : EIF3F eukaryotic translation initiation factor 3, subunit F [ Homo sapiens ]
Official Symbol : EIF3F
Synonyms : EIF3F; eukaryotic translation initiation factor 3, subunit F; EIF3S5, eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDa; eukaryotic translation initiation factor 3 subunit F; eIF3 epsilon; eIF3 p47; eIF3f; eIF3-epsilon; eIF-3-epsilon; eukaryotic translation initiation factor 3, subunit 5 epsilon, 47kDa; eukaryotic translation initiation factor 3, subunit 5 (epsilon, 47kD); EIF3S5; eIF3-p47;
Gene ID : 8665
mRNA Refseq : NM_003754
Protein Refseq : NP_003745
MIM : 603914
UniProt ID : O00303
Chromosome Location : 11p15.4
Pathway : Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S, organism-specific biosystem; Cap-dependent Translation Initiation, organism-specific biosystem; Eukaryotic Translation Initiation, organism-specific biosystem; Formation of a pool of free 40S subunits, organism-specific biosystem; Formation of the ternary complex, and subsequently, the 43S complex, organism-specific biosystem; GTP hydrolysis and joining of the 60S ribosomal subunit, organism-specific biosystem; Gene Expression, organism-specific biosystem;
Function : protein binding; contributes_to translation initiation factor activity; contributes_to translation initiation factor activity; translation initiation factor activity;

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