Recombinant Human coiled-coil-helix-coiled-coil-helix domain containing 3, His-tagged

• Cat.No.: CHCHD3-295H
• Product Overview: CHCHD3, 1-227aa, Human, His tag, E.coli

Specification

• Description: Coiled-coil-helix-coiled-coil-helix domain containing protein 3, also known as CHCHD3, is required for maintenance of mitochondrial crista integrity and mitochondrial function. This protein may act as a scaffolding protein that stabilizes protein complexes involved in crista architecture and protein import. It has also been shown to function as a transcription factor which binds to the BAG1 promoter and represses BAG1 transcription. Recombinant human CHCHD3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Source: E.coli
• Species: Human
• Tag: His-tag
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT
• Molecular Mass: 28.5 kDa (250aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMGGTTST RRVTFEADEN ENITVVKGIR LSENVIDRMK ESSPSGSKSQ RYSGAYGASV SDEELKRRVA EELALEQAKK ESEDQKRLKQ AKELDRERAA ANEQLTRAIL RERICSEEER AKAKHLARQL EEKDRVLKKQ DAFYKEQLAR LEERSSEFYR VTTEQYQKAA EEVEAKFKRY ESHPVCADLQ AKILQCYREN THQTLKCSAL ATQYMHCVNH AKQSMLEKGG
• Purity: >80% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25mg/ml (determined by Bradford assay)
• Publication:
  Global Interactome Mapping of Mitochondrial Intermembrane Space Proteases Identifies a Novel Function for HTRA2 (2019)
  Interactome Mapping of the Mitochondrial Intermembrane Space Proteases Identifies a Novel Function of HTRA2 (2020)

Gene Information

• Gene Name: CHCHD3 coiled-coil-helix-coiled-coil-helix domain containing 3 [ Homo sapiens ]
• Official Symbol: CHCHD3
• Synonyms: CHCHD3; coiled-coil-helix-coiled-coil-helix domain containing 3; coiled-coil-helix-coiled-coil-helix domain-containing protein 3, mitochondrial; FLJ20420; MINOS3; mitochondrial inner membrane organizing system 3; PPP1R22; protein phosphatase 1; regulatory subunit 22; protein phosphatase 1, regulatory subunit 22
• Gene ID: 54927
• mRNA Refseq: NM_017812
• Protein Refseq: NP_060282
• MIM: 613748
• UniProt ID: Q9NX63
• Chromosome Location: 7q33
• Function: phosphatase binding; protein binding; protein complex scaffold