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Recombinant Human acetylserotonin O-methyltransferase, His-tagged

Cat.No.: ASMT-350H
Product Overview: ASMT, 1-298aa, Human, His tag, E.coli
Description: ASMT belongs to the methyltransferase superfamily and is involved in melatonin biosynthesis. Expressed in brain, retina and pineal gland, ASMT functions to catalyze the final reaction in the synthesis of melatonin, specifically the conversion of S-adenosyl-L-methionine and N-acetylserotonin to S-adenosyl-L-homocysteine and melatonin. Recombinant human ASMT protein, fused to His-tag at N-terminus, was expressed in E.coli.
Source: E.coli
Species: Human
Tag: His-tag
Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1M Urea, 10% glycerol
Molecular Mass: 35.3 kDa (318aa)
AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSSEDQAYR LLNDYANGFM VSQVLFAACE LGVFDLLAEA PGPLDVAAVA AGVRASAHGT ELLLDICVSL KLLKVETRGG KAFYRNTELS SDYLTTVSPT SQCSMLKYMG RTSYRCWGHL ADAVREGRNQ YLETFGVPAE ELFTAIYRSE GERLQFMQAL QEVWSVNGRS VLTAFDLSVF PLMCDLGGDF FKDPLPEADL YILARVLHDW ADGKCSHLLE RIYHTCKPGG GILVIESLLD EDRRGPLLTQ LYSLNMLVQT EGQERTPTHY HMLLSSAGFR DFQFKKTGAI YDAILARK
Purity: >90% by SDS - PAGE
Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Concentration: 1 mg/ml (determined by Bradford assay)
Gene Name: ASMT acetylserotonin O-methyltransferase [ Homo sapiens ]
Official Symbol: ASMT
Synonyms: ASMT; acetylserotonin O-methyltransferase; ASMTY; HIOMT; HIOMTY; hydroxyindole O-methyltransferase; acetylserotonin N-methyltransferase; acetylserotonin methyltransferase (Y chromosome);
Gene ID: 438
mRNA Refseq: NM_001171038
Protein Refseq: NP_001164509
MIM: 300015
UniProt ID: P46597
Chromosome Location: Xp22.3 and Yp11.3
Pathway: Amine-derived hormones, organism-specific biosystem; Biogenic Amine Synthesis, organism-specific biosystem; Melatonin biosynthesis, tryptophan =>serotonin => melatonin, organism-specific biosystem; Melatonin biosynthesis, tryptophan => serotonin =>
Function: O-methyltransferase activity; acetylserotonin O-methyltransferase activity; transferase activity;

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