"ansA" Related Products

Recombinant E.coli cytoplasmic L-asparaginase I, His-tagged

Cat.No.: ansA-698E
Product Overview: ansA, 1-338aa, E.coli, His tag, E.coli
Description: AnsA is the cytoplasmic asparaginase from Escherichia coli involved in intracellular asparagine utilization. Escherichia coli possesse two l-asparaginases: the cytoplasmic type I form (ansA) and the periplasmic type II form (ansB). AnsA (Type I asparaginase) is constitutively expressed and required for the growth of the bacteria on asparagine as the sole nitrogen source. Recombinant E.coli ansA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Source: E.coli
Species: E.coli
Tag: His-tag
Form: Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 2mM DTT.
Molecular Mass: 39.3 kDa (358aa), confirmed by MALDI-TOF
AA Sequence: MGSSHHHHHH SSGLVPRGSH MQKKSIYVAY TGGTIGMQRS EQGYIPVSGH LQRQLALMPE FHRPEMPDFT IHEYTPLMDS SDMTPEDWQH IAEDIKAHYD DYDGFVILHG TDTMAYTASA LSFMLENLGK PVIVTGSQIP LAELRSDGQI NLLNALYVAA NYPINEVTLF FNNRLYRGNR TTKAHADGFD AFASPNLPPL LEAGIHIRRL NTPPAPHGEG ELIVHPITPQ PIGVVTIYPG ISADVVRNFL RQPVKALILR SYGVGNAPQN KAFLQELQEA SDRGIVVVNL TQCMSGKVNM GGYATGNALA HAGVIGGADM TVEATLTKLH YLLSQELDTE TIRKAMSQNL RGELTPDD
Purity: >95% by SDS - PAGE
Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Concentration: 0.5mg/ml (determined by Bradford assay)
Gene Name: ansA?cytoplasmic L-asparaginase I [?Escherichia coli str. K-12 substr. W3110?]
Official Symbol: ansA
Synonyms: ansA; cytoplasmic L-asparaginase I; YP_490028.1; ECK1765:JW1756:b1767
Gene ID: 12930147
Protein Refseq: YP_490028
UniProt ID: AP_002386
Pathway: Alanine, aspartate and glutamate metabolism; Biosynthesis of secondary metabolites; Cyanoamino acid metabolism

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