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Recombinant Bacillus subtilis ANSA protein, His-tagged

Cat.No. : ANSA-0911B
Product Overview : Recombinant Bacillus subtilis ANSA full length or partial length protein was expressed.
  • Specification
  • Gene Information
  • Related Products
Source : E. coli or Yeast
Species : Bacillus subtilis
Tag : His
Form : Liquid or lyophilized powder
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : > 80% by SDS-PAGE
Notes : This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
Storage : Store it at +4 ºC for short term. For long term storage, store it at -20 ºC~-80 ºC.
Storage buffer : PBS buffer
Gene Name : ansA L-asparaginase 1 [ Bacillus subtilis subsp. subtilis str. 168 ]
Official Symbol : ANSA
Synonyms : ANSA; L-asparaginase 1
Gene ID : 938722
UniProt ID : P26900

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (18)

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Can ansA protein be targeted for drug development? 08/21/2022

Yes, ansA protein has been considered as a potential target for drug development, particularly in the field of antimicrobial agents. Synthetic analogs or inhibitors of ansA have been designed and tested to hinder PABA biosynthesis and folate production in bacteria, as this would impair their growth and replication. These inhibitors may have therapeutic potential as antimicrobial agents against bacterial infections.

Is there a correlation between ansA expression and antibiotic resistance? 08/04/2022

There is currently limited evidence of a direct correlation between ansA gene expression and antibiotic resistance. However, ansA is crucial for folate synthesis, which plays a role in DNA synthesis and repair. It is possible that bacteria with higher ansA expression may have enhanced DNA repair mechanisms, potentially mitigating the effects of some antibiotics that target DNA replication or repair processes. Further research is needed to explore this correlation in detail.

Are there any natural compounds that can modulate ansA activity? 03/20/2021

since the ansA gene is involved in the biosynthesis of p-aminobenzoate (PABA), compounds that affect PABA availability or metabolism could indirectly impact ansA activity. For example, antibiotics like sulfonamides and trimethoprim target bacterial enzymes involved in folate synthesis, which can lead to depletion of PABA and subsequently affect ansA function.

What happens if the ansA gene is knocked out or deleted? 01/25/2021

If the ansA gene is knocked out or deleted, bacteria would lose the ability to synthesize p-aminobenzoate (PABA), which is a key precursor for the synthesis of folate. As a consequence, the bacteria would be unable to produce sufficient amounts of folate, which is essential for essential cellular processes such as DNA synthesis and repair. This could result in growth defects or even cell death in certain conditions.

Is the ansA protein a target for antimicrobial agents? 10/11/2020

Yes, the ansA protein has been targeted by certain antibiotics and antimicrobial agents. Inhibitors that specifically interfere with the activity of ansA have been developed to disrupt PABA synthesis in bacteria and inhibit their folate production, which is essential for their growth and survival.

Are there any known genetic polymorphisms or mutations in the ansA gene? 02/28/2020

Information on genetic polymorphisms or mutations in the ansA gene is limited. Most studies have focused on functional characterization and expression analysis of ansA rather than genetic variation. However, with advancements in sequencing technologies, it is possible that future studies may identify genetic polymorphisms or mutations in the ansA gene across different bacterial species or strains. These genetic variations could have implications for ansA function and bacterial physiology.

How is ansA gene expression regulated in bacteria? 02/17/2020

The regulation of ansA gene expression in bacteria is not well-characterized. However, some studies suggest that ansA expression can be regulated at the transcriptional level. For example, in Escherichia coli, the ansA gene is negatively regulated by a repressor protein called AnsR, which binds to specific sequences in the ansA promoter region. Under low PABA conditions, AnsR dissociates from the promoter, allowing for increased ansA transcription. Other factors, such as nutrient limitation or exposure to stressors, may also influence ansA expression, although the specific regulatory mechanisms remain to be elucidated.

Can ansA protein be used for biotechnological applications? 12/23/2019

The ansA protein itself may not have direct biotechnological applications. However, the knowledge gained from studying the protein and its role in PABA biosynthesis can be used in biotechnological processes involving folate production or engineering PABA production pathways in bacteria. Additionally, the inhibitors or molecules targeting ansA protein developed for antimicrobial purposes may have broader applications in various biotechnological fields.

Is there any research on ansA gene expression in different bacterial species? 11/24/2019

Research on the expression of the ansA gene in different bacterial species is limited. Most studies have focused on its expression and regulation in model organisms or bacteria of medical importance. However, with the advent of high-throughput sequencing techniques, it is becoming easier to explore gene expression patterns across diverse bacterial species, including ansA. This can provide insights into the conservation or variation of ansA regulation in different bacteria.

Can ansA be a target for developing novel antibiotics? 08/08/2019

Targeting the ansA gene or the pathway it is involved in could potentially be a strategy for developing novel antibiotics. Since ansA is essential for the biosynthesis of PABA and, subsequently, folate, inhibiting or modulating its activity could disrupt bacterial growth and survival. However, further research is needed to understand the feasibility and potential side effects of targeting ansA specifically for antibiotic development. Additionally, considering the essential role of folate metabolism in both bacteria and human cells, careful consideration of selective toxicity would be crucial.

Can ansA gene expression be induced under specific conditions? 04/27/2018

The ansA gene expression can be induced under specific conditions, particularly when bacteria are in environments lacking or limited in external sources of p-aminobenzoate (PABA). In such conditions, bacteria may upregulate the ansA gene to ensure sufficient PABA biosynthesis for folate production. Other stress conditions, such as nutrient deprivation or exposure to antibiotics, may also trigger ansA expression as a protective response.

Are there any known genetic mutations or polymorphisms in the ansA gene? 04/21/2018

Genetic mutations or polymorphisms in the ansA gene have been reported in some bacteria. These mutations can impact the activity or stability of the ansA protein, leading to changes in PABA biosynthesis and potentially affecting bacterial survival or drug resistance.

Can the ansA protein be regulated or modulated in any way? 11/29/2017

Regulation of the ansA protein can occur at the transcriptional level in response to environmental cues. Some studies have identified specific transcription factors and regulatory elements that control the expression of the ansA gene.

Are there any known diseases or disorders associated with ansA protein dysfunction? 07/21/2017

Since the ansA protein is primarily found in bacteria and plays a crucial role in folate synthesis, disruptions in its function can impact bacterial growth and survival. However, there are no known human diseases or disorders directly associated with ansA protein dysfunction.

Are there any inhibitors or activators of ansA activity that have been identified? 01/27/2017

The identification of specific inhibitors or activators of ansA activity is relatively limited. However, certain compounds have been shown to affect ansA expression or function indirectly. For example, sulfonamides and trimethoprim are antibiotics that target enzymes involved in folate synthesis, leading to depletion of PABA and potentially impacting ansA activity.

Can ansA protein be found in eukaryotic cells? 01/23/2017

The ansA protein is primarily found in bacteria and has not been reported in eukaryotic cells. The biosynthesis of folate, which requires PABA, is different in eukaryotes compared to bacteria. Eukaryotes typically acquire folate through dietary sources instead of synthesizing it de novo like bacteria do.

Are there any known interacting proteins or cofactors for ansA? 09/29/2016

The ansA protein may interact with other proteins or cofactors within the biosynthetic pathway of PABA production or folate synthesis. However, specific interacting partners have not been extensively characterized or identified. Further research is needed to understand the protein interactions involved in PABA biosynthesis pathway and its regulation.

Can the ansA gene be used as a genetic marker or diagnostic tool? 09/10/2016

The ansA gene may not be commonly used as a specific genetic marker or diagnostic tool on its own. However, the biosynthesis pathway it is involved in, along with other genes related to folate metabolism, can be collectively used as markers or targets for diagnostic tests. For example, detection of specific gene variants or mutations in these pathways can indicate susceptibility to certain diseases or help monitor the effectiveness of drug treatments targeting folate metabolism.

Customer Reviews (5)

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Reviews
08/25/2022

    Its exceptional purity and integrity ensure clear and reliable bands, enabling accurate detection and quantification of target proteins.

    02/19/2022

      the ansA Protein's applicability in protein electron microscopy structure analysis is remarkable.

      07/23/2021

        ts stability and structural integrity make it an ideal candidate for studying protein structures at high resolution.

        02/01/2020

          In WB experiments, the ansA Protein consistently delivers exceptional performance.

          06/21/2017

            This protein has consistently demonstrated outstanding results, making it an indispensable tool in my research endeavors.

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