Recombinant Human BRCA2 Protein, His&GST-tagged

• Cat.No.: BRCA2-232H
• Product Overview: Recombinant Human BRCA2 Protein(AAH09771.1) (Met1-Val314) was expressed in Insect Cells, with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: 1-314 aa
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 3mM DTT, 10% glycerol, pH 7.4.
• Molecular Mass: The recombinant human BCCIP/GST chimera consists of 551 amino acids and has a calculated molecular mass of 64 kDa. The recombinant protein migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE.
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: BRCA2 breast cancer 2, early onset [ Homo sapiens ]
• Official Symbol: BRCA2
• Synonyms: BRCA2; breast cancer 2, early onset; FACD, FANCD, FANCD1, Fanconi anemia, complementation group D1; breast cancer type 2 susceptibility protein; BRCA1/BRCA2 containing complex; subunit 2; BRCC2; FAD; FAD1; fanconi anemia group D1 protein; breast cancer 2 tumor suppressor; BRCA1/BRCA2-containing complex, subunit 2; breast cancer susceptibility protein BRCA2; breast and ovarian cancer susceptibility gene, early onset; FACD; GLM3; FANCB; FANCD; PNCA2; FANCD1; BROVCA2
• Gene ID: 675
• mRNA Refseq: NM_000059
• Protein Refseq: NP_000050
• MIM: 600185
• UniProt ID: P51587