Recombinant Human BRCA2, His & GST-tagged

• Cat.No.: BRCA2-232H
• Product Overview: A DNA sequence encoding the human BCCIP (AAH09771.1) (Met1-Val314) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Source: Baculovirus-Insect Cells
• Species: Human
• Tag: His & GST
• Form: Lyophilized from sterile PBS, pH7.4.
• Molecular Mass: The recombinant human BCCIP/GST chimera consists of 551 amino acids and has a calculated molecular mass of 64 kDa. The recombinant protein migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
• Protein length: Met1-Val314
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >85 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: Hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: BRCA2 breast cancer 2, early onset [ Homo sapiens ]
• Official Symbol: BRCA2
• Synonyms: BRCA2; breast cancer 2, early onset; FACD, FANCD, FANCD1, Fanconi anemia, complementation group D1; breast cancer type 2 susceptibility protein; BRCA1/BRCA2 containing complex; subunit 2; BRCC2; FAD; FAD1; fanconi anemia group D1 protein; breast cancer 2 tumor suppressor; BRCA1/BRCA2-containing complex, subunit 2; breast cancer susceptibility protein BRCA2; breast and ovarian cancer susceptibility gene, early onset; FACD; GLM3; FANCB; FANCD; PNCA2; FANCD1; BROVCA2
• Gene ID: 675
• mRNA Refseq: NM_000059
• Protein Refseq: NP_000050
• MIM: 600185
• UniProt ID: P51587
• Chromosome Location: 13q12-q13
• Pathway: DNA Repair, organism-specific biosystem; Double-Strand Break Repair, organism-specific biosystem; FOXM1 transcription factor network, organism-specific biosystem; Fanconi Anemia pathway, organism-specific biosystem; Fanconi anemia pathway, organism-specific biosystem; Fanconi anemia pathway, conserved biosystem; Homologous DNA pairing and strand exchange, organism-specific biosystem
• Function: DNA binding; H3 histone acetyltransferase activity; H4 histone acetyltransferase activity; gamma-tubulin binding; NOT histone acetyltransferase activity; protease binding; protein binding; single-stranded DNA binding