||Recombinant full-length human HUNK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
||HUNK or hormonally up-regulated Neu-associated kinase is a member of the mitogen-activated protein kinase which is essential for mammary tumor metastasis. Hunk deletion in mice did not alter viability, fertility, longevity, or propensity to develop tumors and it is also dispensable for murine development.
||Sf9 insect cells using baculovirus
||50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
||Kinase Assay, Western Blot
||Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.