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Recombinant Human ASAP2, His-tagged

Cat.No. : ASAP2-3618H
Product Overview : Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 (ASAP2), partial
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Description : This gene encodes a multidomain protein containing an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology (PH) domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal Src homology 3 (SH3) domain. The protein localizes in the Golgi apparatus and at the plasma membrane, where it colocalizes with protein tyrosine kinase 2-beta (PYK2). The encoded protein forms a stable complex with PYK2 in vivo. This interaction appears to be mediated by binding of its SH3 domain to the C-terminal proline-rich domain of PYK2. The encoded protein is tyrosine phosphorylated by activated PYK2. It has catalytic activity for class I and II ArfGAPs in vitro, and can bind the class III Arf ARF6 without immediate GAP activity. The encoded protein is believed to function as an ARF GAP that controls ARF-mediated vesicle budding when recruited to Golgi membranes. In addition, it functions as a substrate and downstream target for PYK2 and SRC, a pathway that may be involved in the regulation of vesicular transport. Multiple transcript variants encoding different isoforms have been found for this gene.
Source : E. Coli or Yeast
Species : Human
Tag : His
Form : This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
Protein length : 961
Purity : >90%
Notes : Small volumes of ASAP2 recombinant protein may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice.
Storage : Store at -20 degree C. For extended storage, store at -20 or -80 degree C.
Storage Buffer : PBS pH 7.4, 50% glycerol
Warning : This product is for research use only. Not for use in diagnostic or therapeutic procedures.
Gene Name : ASAP2 ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 [ Homo sapiens ]
Official Symbol : ASAP2
Synonyms : ASAP2; ArfGAP with SH3 domain, ankyrin repeat and PH domain 2; DDEF2, development and differentiation enhancing factor 2; arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2; centaurin; beta 3; CENTB3; KIAA0400; PAP; SHAG1; centaurin, beta 3; PYK2 C terminus-associated protein; pyk2 C-terminus-associated protein; development and differentiation enhancing factor 2; development and differentiation-enhancing factor 2; paxillin-associated protein with ARF GAP activity 3; PAG3; AMAP2; DDEF2; Pap-alpha; FLJ42910;
Gene ID : 8853
mRNA Refseq : NM_001135191
Protein Refseq : NP_001128663
MIM : 603817
UniProt ID : O43150
Chromosome Location : 2p24
Pathway : Arf6 trafficking events, organism-specific biosystem; Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem; Fc gamma R-mediated phagocytosis, organism-specific biosystem; Fc gamma R-mediated phagocytosis, conserved biosystem;
Function : ARF GTPase activator activity; enzyme activator activity; metal ion binding; protein binding; zinc ion binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (8)

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Where is ASAP2 primarily localized within cells? 12/04/2022

ASAP2 is found in the cytoplasm, associated with cellular membranes, particularly the Golgi apparatus and endosomes.

Can ASAP2 be regulated by post-translational modifications? 10/18/2022

Yes, ASAP2 can undergo phosphorylation and ubiquitination, which may influence its stability, activity, and protein-protein interactions.

Can ASAP2 interact with other proteins? 05/17/2021

Yes, ASAP2 can interact with various proteins involved in membrane trafficking, including clathrin, AP1, AP3, and many others.

Is ASAP2 involved in cancer progression? 04/25/2020

ASAP2 has been implicated in cancer metastasis due to its ability to promote cell migration and invasion through its effect on the actin cytoskeleton.

How does ASAP2 regulate membrane trafficking? 01/06/2020

ASAP2 promotes the hydrolysis of GTP bound to Arf1, leading to the inactivation of Arf1 and subsequent modulation of vesicle budding, cargo sorting, and membrane remodeling.

What are the domains present in ASAP2? 09/21/2018

ASAP2 contains an SH3 domain, an ANK repeat domain, and a PH (pleckstrin homology) domain.

Are there any genetic variations associated with ASAP2? 05/25/2018

Genetic variations in ASAP2 have been identified in some studies, and they may be linked to certain diseases or phenotypes, although further research is needed to establish concrete associations.

Does ASAP2 play a role in cell adhesion? 01/31/2017

Yes, ASAP2 has been suggested to modulate cell adhesion and migration by regulating actin cytoskeleton dynamics through its interaction with Rho family GTPases.

Customer Reviews (3)

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Reviews
05/23/2022

    The ASAP2 protein exhibits outstanding performance in enzyme-linked immunosorbent assays (ELISA).

    11/13/2021

      The protein's reliability and reproducibility in WB ensure accurate analysis of target proteins, while its suitability for electron microscopy structure analysis enables detailed examination of molecular structures.

      11/16/2020

        The versatility and efficacy of ASAP2 in these applications make it an invaluable tool for researchers.

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