"PTA" Related Products

Native Bacillus stearothermophilus Phosphotransacetylase

Cat.No.: PTA-23B
Product Overview: Native Bacillus stearothermophilus Acetate Kinase was purified from Bacillus stearothermophilus.
Description: Acetate kinase plays an important role in glycolysis. Acetate kinase phosphorylates acetate in the presence of ATP and a divalent cation, which ultimately results in the production of acetyl-CoA. Acetate kinase is also involved in the metabolism of propanoate, pyruvate and taurine. Acetate Kinase from Bacillus stearothermophilus is a thermostable tetramer of identical subunits with molecular weight of 40,000 Da each. The enzyme does not have a -SH group and is composed of 36% β-structure, 21 % α-helix and 43 % unordered structure.
Source: Bacillus stearothermophilus
Species: Bacillus stearothermophilus
Form: Lyophilized powder containing potassium phosphate.
Bio-activity: Involved in the metabolism of propanoate, pyruvate and taurine. 400-1,200 units/mg solid.
Applications: Acetate kinase is used to phosphorylate acetate to acetyl phosphate. Acetate Kinase from Bacillus stearothermophilus has been used to study allosteric activation. [32P]-acetyl phosphate was generated by incubating potassium acetate in the reaction mixture with acetate kinase from Sigma. This [32P]-acetyl phosphate was used to label BldM, BldM D-54N or BldM D-54A loci during the study of the effect of bldM gene on Streptomyces coelicolor development.
Storage: 2-8°C.

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