"IMAC" Related Products

IMAC Agarose Fast Flow

Cat.no: IMAC FF-001A
Product Overview: IMAC agarose Fast Flow is a BioProcess IMAC medium, designed for purifying proteins and peptides that have an affinity for metal ions, such as histidine-tagged proteins but also untagged recombinant or native proteins.
Characteristics: IMAC agarose Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix. IMAC agarose 6 Fast Flow is supplied free of metal ions, allowing the user to charge it with the most appropriate metal ion for purification of a target protein.The coupling chemistry and the novel ligand is designed to give minimal ligand leakage while providing high capacity.IMAC agarose 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
Size: 25 mL, 100 mL
Matrix: Highly cross-linked 6% spherical agarose
Ion exchange capacity: Approx. 15 μmol Ni2+/ml medium
Average particle size: 90μm
Dynamic binding capacity: Approx. 40 mg (histidine)6-tagged protein/ml medium (Ni2+ charged).
Recommended flow rate: <150>
Recommended column height: 5‐20cm
Chemical stability: Stable in: 0.01 M HCl, 0.1 M NaOH. Tested for one week at 40°C. 1 M NaOH, 70% acetic acid. Tested for 12 h.2% SDS. Tested for 1 h. 30% 2-propanol. Tested for 30 min.
pH working range: 3-12
pH CIP range: 2-14
Temperature Stability: 4-30°C
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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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