"IMAC" Related Products

      IMAC Agarose Fast Flow

      Cat.no : IMAC FF-001A
      Product Overview : IMAC agarose Fast Flow is a BioProcess IMAC medium, designed for purifying proteins and peptides that have an affinity for metal ions, such as histidine-tagged proteins but also untagged recombinant or native proteins.
      Characteristics : IMAC agarose Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix. IMAC agarose 6 Fast Flow is supplied free of metal ions, allowing the user to charge it with the most appropriate metal ion for purification of a target protein.The coupling chemistry and the novel ligand is designed to give minimal ligand leakage while providing high capacity.IMAC agarose 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
      Size : 25 mL, 100 mL
      Matrix : Highly cross-linked 6% spherical agarose
      Ion exchange capacity : Approx. 15 μmol Ni2+/ml medium
      Average particle size : 90μm
      Dynamic binding capacity : Approx. 40 mg (histidine)6-tagged protein/ml medium (Ni2+ charged).
      Recommended flow rate : <150>
      Recommended column height : 5‐20cm
      Chemical stability : Stable in: 0.01 M HCl, 0.1 M NaOH. Tested for one week at 40°C. 1 M NaOH, 70% acetic acid. Tested for 12 h.2% SDS. Tested for 1 h. 30% 2-propanol. Tested for 30 min.
      pH working range : 3-12
      pH CIP range : 2-14
      Temperature Stability : 4-30°C
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