IMAC Agarose Fast Flow
| Cat.No. : | IMAC FF-001A |
| Product Overview : | IMAC agarose Fast Flow is a BioProcess IMAC medium, designed for purifying proteins and peptides that have an affinity for metal ions, such as histidine-tagged proteins but also untagged recombinant or native proteins. |
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| Cat.no : | IMAC FF-001A |
| Characteristics : | IMAC agarose Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix. IMAC agarose 6 Fast Flow is supplied free of metal ions, allowing the user to charge it with the most appropriate metal ion for purification of a target protein.The coupling chemistry and the novel ligand is designed to give minimal ligand leakage while providing high capacity.IMAC agarose 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. |
| Size : | 25 mL, 100 mL |
| Matrix : | Highly cross-linked 6% spherical agarose |
| Ion exchange capacity : | Approx. 15 μmol Ni2+/ml medium |
| Average particle size : | 90μm |
| Dynamic binding capacity : | Approx. 40 mg (histidine)6-tagged protein/ml medium (Ni2+ charged). |
| Recommended flow rate : | <150> |
| Recommended column height : | 5‐20cm |
| Chemical stability : | Stable in: 0.01 M HCl, 0.1 M NaOH. Tested for one week at 40°C. 1 M NaOH, 70% acetic acid. Tested for 12 h.2% SDS. Tested for 1 h. 30% 2-propanol. Tested for 30 min. |
| pH working range : | 3-12 |
| pH CIP range : | 2-14 |
| Temperature Stability : | 4-30°C |
Products Types
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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