"IMAC" Related Products

IMAC Agarose High Performance

Cat.no: IMAC HP-001A
Product Overview: IMAC agarose High Performance media is easily charged by the user with the metalion of choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.
Characteristics: 1. For optimizing purification of histidine-tagged proteins when Ni2+ is not the best choice of metal ion2. Conveniently charge with your metal of choice3. 34 μm bead size for high performance purification4. High binding capacity
Size: 25 mL
Matrix: Highly cross-linked agarose, 6%
Ion exchange capacity: Approx. 15 μmol Ni2+/ml medium
Average particle size: 34μm
Dynamic binding capacity: At least 40 mg histidine-tagged protein/ml medium when changed with Ni2?
Recommended flow rate: <300>
Recommended column height: 5‐20cm
Chemical stability: Stable in all commonly used buffers, At 40°Cc0.01 M HCl, 0.1 M NaOH [For One Week] 1M NaOH, 70% acetic acid [For 12 h]30% 2-propanol [30 min] 2% SDS [1 h]
pH working range: 3-12
pH CIP range: 2-14
Temperature Stability: 4-30°C
Warning: Avoid using Chelating agents, e.g. EDTA, EGTA, citrate
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