"Chelate" Related Products

Chelating Agarose Fast Flow

Cat.no: Chelate FF-001A
Product Overview: Chelating agarose Fast Flow is a BioProcess IMAC medium, designed for purifying native and recombinant proteins and peptides that have an affinity for metal ions.
Characteristics: Chelating agarose Fast Flow is composed of cross-linked 6% agarose beads modified with iminodiacetic immobilized to the base matrix by stable ether linkages and sufficiently long spacer arms.Chelating agarose Fast Flow is supplied free of metal ions, allowing the user to charge it with the most appropriate metal ion for purification of a target protein.Chelating agarose Fast Flow is available in a range of different bulk pack sizes for easy scale-up and process development. As member of the BioProcess media range, Chelating agarose Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
Size: 50 mL
Matrix: Highly cross-linked agarose, 6%
Average particle size: 90μm
Ligand: Iminodiacetic acid
Ligand concentration: 30–37 μmol Cu2+/ml drainedmedium
Recommended flow rate: 200-450 cm/h
Recommended column height: 5‐20cm
Chemical stability: Stable in commonly used aqueous buffers - 1 M NaOH, 20% ethanol, 8 M guanidine hydrochloride, 8 M urea, 0.01 M HCl, non-ionic detergents, acetate buffer pH 4
pH working range: 3-13
pH CIP range: 2-14
Temperature Stability: 4-30°C
Autoclavable: In 0.1 M sodium acetate at 121 °C for 30 min
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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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