"Thiopropyl" Related Products

Thiopropyl Agarose

Cat.no: Thio-001A
Product Overview: Thiopropyl Sepharos contains reactive 2-thiopyridyl disulphide groups attached to agarose through a chemically stable ether linkage, which reacts with solutes containing thiol groups under mild conditions to form mixed disulphides. This reaction forms the basis of covalent chromatography and a procedure for reversible immobilization of thiol containing molecules.
Characteristics: 1. separate thiol-containing proteins and peptides from non-thiol-containing proteins and peptides2. in certain cases separate enzymes with active-site thiol groups from denatured enzymes3. store and protect thiol-containing proteins4. regenerate both the coupled biomolecule and the active bed material
Size: 15 g
Matrix: 6% agarose
Functional group: 2-pyridyl disulphide
Ligand concentration: 18–31 μmole 2-pyridyl disulphide/ml drained medium
Coupling chemistry: approx 10–20 mg protein/ml drained medium, e.g. 14 mg ceruloplasmin (Mr 132 000) /ml drained medium
Recommended column height: 5‐20cm
Chemical stability: Stable to all commonly used aqueous buffers and additives like detergents. Azides should be avoided.
Physical stability: Negligible volume variation due to changes in pH or ionic strength.
pH working range: 2-8
pH CIP range: 2-8

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.