"Thiopropyl" Related Products

      Thiopropyl Agarose

      Cat.no : Thio-001A
      Product Overview : Thiopropyl Sepharos contains reactive 2-thiopyridyl disulphide groups attached to agarose through a chemically stable ether linkage, which reacts with solutes containing thiol groups under mild conditions to form mixed disulphides. This reaction forms the basis of covalent chromatography and a procedure for reversible immobilization of thiol containing molecules.
      Characteristics : 1. separate thiol-containing proteins and peptides from non-thiol-containing proteins and peptides2. in certain cases separate enzymes with active-site thiol groups from denatured enzymes3. store and protect thiol-containing proteins4. regenerate both the coupled biomolecule and the active bed material
      Size : 15 g
      Matrix : 6% agarose
      Functional group : 2-pyridyl disulphide
      Ligand concentration : 18–31 μmole 2-pyridyl disulphide/ml drained medium
      Coupling chemistry : approx 10–20 mg protein/ml drained medium, e.g. 14 mg ceruloplasmin (Mr 132 000) /ml drained medium
      Recommended column height : 5‐20cm
      Chemical stability : Stable to all commonly used aqueous buffers and additives like detergents. Azides should be avoided.
      Physical stability : Negligible volume variation due to changes in pH or ionic strength.
      pH working range : 2-8
      pH CIP range : 2-8

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