Thiopropyl Agarose

• Cat.No.: Thio-001A
• Product Overview: Thiopropyl Sepharos contains reactive 2-thiopyridyl disulphide groups attached to agarose through a chemically stable ether linkage, which reacts with solutes containing thiol groups under mild conditions to form mixed disulphides. This reaction forms the basis of covalent chromatography and a procedure for reversible immobilization of thiol containing molecules.

Specification

• Cat.no: Thio-001A
• Characteristics: 1. separate thiol-containing proteins and peptides from non-thiol-containing proteins and peptides2. in certain cases separate enzymes with active-site thiol groups from denatured enzymes3. store and protect thiol-containing proteins4. regenerate both the coupled biomolecule and the active bed material
• Size: 15 g
• Matrix: 6% agarose
• Functional group: 2-pyridyl disulphide
• Ligand concentration: 18–31 μmole 2-pyridyl disulphide/ml drained medium
• Coupling chemistry: approx 10–20 mg protein/ml drained medium, e.g. 14 mg ceruloplasmin (Mr 132 000) /ml drained medium
• Recommended column height: 5‐20cm
• Chemical stability: Stable to all commonly used aqueous buffers and additives like detergents. Azides should be avoided.
• Physical stability: Negligible volume variation due to changes in pH or ionic strength.
• pH working range: 2-8
• pH CIP range: 2-8
• Publication:
  The role of an amphiphilic helix and transmembrane region in the efficient acylation of the M2 protein from influenza virus (2023)